白屈菜红碱对变形链球菌细胞形态学影响的透射电镜观察  

Transmission Electron Microscopic Observation of Morphological Influence of Chelerythrine on Streptococcus Mutans

作　　者：徐冬雪[1] 陈旭[2] 程睿波[3] 

机构地区：[1]广州医学院口腔医院口腔内科,广东广州510140 [2]中国医科大学口腔医学院儿童口腔科 [3]中国医科大学口腔医学院口腔预防科

出　　处：《口腔医学研究》2011年第10期870-872,共3页Journal of Oral Science Research

基　　金：辽宁省自然科学基金(编号:20072089)

摘　　要：目的:通过透射电镜(transmission electron microscope,TEM)观察白屈菜红碱(chelerythrine,CHE)对变形链球菌(streptococcus mutans,S.mutans)作用后的形态学影响,探讨其抑菌机制。方法:选择变形链球菌ATCC25175作为实验菌株,实验分白屈菜红碱溶液处理组(实验组)及对照组,将2组细菌在37℃厌氧环境(80%N2、20%CO2)下培养24h,透射电镜观察细菌细胞结构的变化。结果:白屈菜红碱通过抑制变形链球菌的细胞分裂而抑制其生长,并对荚膜、细胞壁、细胞膜等结构产生显著影响,使其完整性受到破坏。结论:白屈菜红碱可以改变变形链球菌超微结构,这可能是其抑制细菌生长的机制之一。Objective： To investigate the effect of chelerythrine on morphology of streptococcus mutans（S.mutans） by transmission electron microscope（TEM）,and to evaluate its potential antibacterial mechanism.Methods： S.mutans ATCC25175 was chosen as the experimental bacteria.The study was divided into two groups： chelerythrine solution treatment group and control group.The bacteria were cultured at 37℃ in anaerobic environment（80%N2,20%CO2） for 24 hours,and were observed under TEM.Results： It was found that chelerythrine could inhibit the growth of S.mutans by preventing cell division and causing the defective structure of the capsule,cell wall and cell membrane.Conclusion： Chelerythrine is able to change the super-microscopic structure of S.mutans,and may inhibit the growth of S.mutans partly by this mechanism.

关 键 词：龋病 白屈菜红碱 变形链球菌 透射电镜 

分 类 号：R285[医药卫生—中药学]