益心康对感染柯萨奇病毒B3的大鼠体外心肌细胞CVB_3 mRNA复制及相关细胞因子的影响  

Effect of Yixinkang on reduplication of CVB_3 mRNA of myocardial cells in vitro and correlated cytokines in coxsackievirus-infected rats

作　　者：陈苏宁[1] 马丽[1] 高宪新[1] 

机构地区：[1]中国医科大学附属盛京医院,沈阳110004

出　　处：《天津中医药》2011年第5期410-413,共4页Tianjin Journal of Traditional Chinese Medicine

基　　金：沈阳市科学技术局科研项目(F10-205-1-76)

摘　　要：[目的]通过检测益心康药液作用于感染柯萨奇病毒B3(CVB3)后的乳鼠心肌细胞中柯萨奇病毒mRNA的复制情况及其所释放的肿瘤坏死因子-α(TNF-α)及转化生长因子-β(TGF-β)表达情况,进一步探讨益心康对病毒性心肌炎的病毒复制及相关细胞因子的作用机制。[方法]使益心康的药液作用于乳鼠心肌细胞,用逆转录-聚合酶链反应(RT-PCR)法检测心肌细胞中柯萨奇病毒mRNA的复制情况及其所释放的TNF-α及TGF-β的表达水平。[结果]益心康在CVB3感染的病毒性心肌炎的发病及发展过程中有抑制CVB3复制的作用,同时可以减少相关细胞因子TNF-α、TGF-β的分泌。益心康组(D组)、益心康加地塞米松组(E组)的细胞中的CVB3 mRNA、TNF-α、TGF-β表达水平明显低于病毒感染组(P<0.01),同时低于利巴韦林组(P<0.05),均有统计学意义;D组、E组之间P>0.05,无统计意义。[结论]益心康对柯萨奇病毒感染心肌细胞具有显著保护作用。[Objective] Through detecting the reduplication of coxsackievirus mRNA in myocardial cells of coxsackievirus B3-infected suckling rat received Yixinkang soup and the expression of their released TNF-α and TGF-β the effect mechanism of Yixinkang on the reduplication of CVB3 virus mRNA and the released related cytokines was explored further.[Methods] Yixinkang was exposed to the myocardial cells of rat and then the reduplication of coxsackievirus mRNA in myocardial cells and the expression of released TNF-α and TGF-β were detected with RT-PCR.[Results] Yixinkang could inhibit the reduplication of CVB3 in the pathogenesis and the development process of viral myocarditis.At the same time,it could reduce the secretion of TNF-α,TGF-β.The expression level of CVB3 mRNA,TNF-α and TGF-β in group D and E was much lower than group C（P〈0.01） and B（P〈0.05）.[Conclusion] Yixinkang can significantly protect cardiocytes infected by Coxsackie virus.

关 键 词：益心康 柯萨奇病毒B3 心肌细胞 柯萨奇病毒mRNA复制 

分 类 号：R285.5[医药卫生—中药学]