北五味子的超临界萃取及产物的HPLC-APCI-MS／MS分析  

CO2-SFE Extraction of the Lignans in Schisandra chinensis Fruits and Analysis of Extracting Products by HPLC-APCI-MS/MS

作　　者：宋明杰[1] 邢俊鹏[2] 宋凤瑞[2] 包海鹰[1] 刘志强[2] 刘淑莹[2] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118 [2]中国科学院长春应用化学研究所,吉林长春130022

出　　处：《质谱学报》2011年第5期283-287,共5页Journal of Chinese Mass Spectrometry Society

基　　金：长春市科技发展项目(No.2008256);吉林省科技发展重点项目(No.20090908);吉林省院合作项目(No.2009SYHZ0026)资助

摘　　要：优化超临界流体萃取北五味子中木脂素类成分的提取工艺,对萃取物中所含有效成分的归属进行分析鉴定。以五味子总木脂素为评价指标,采用正交实验法和不同影响因素系统考察法对CO2-SFE萃取北五味子中木脂素类成分提取工艺进行优化,利用液相色谱-大气压化学电离质谱联用法(HPLC-APCI-MS/MS)分析鉴定产物结构。确定了最佳的超临界提取工艺,即压力为25MPa,温度为45℃,提携剂浓度为90%乙醇,并对萃取产物中所含的10种木脂素类化合物进行归属鉴别。证明优化的工艺与其他常规提取工艺相比较科学合理,具有较强的稳定性和有一定的实用价值。The orthogonal test was used to optimize CO2-SFE process of the llgnans m Schisandra chinensis fruits with content of total lignan as index, and the extraction products were analyzed by HPLC-APCI-MS/MS. The optimal extraction procedure is as fol- lows： the extraction pressure is 25 MPa, the extraction temperature is 45 °C ,and the con- centration alcohol is 90%. Ten structures of extraction products are confirmed by HPLC- APCI-MS/MS. The optimum extraction procedure is reasonable and stable. Compared SFE with traditional methods, the optimum extraction procedure is excellent and valuable.

关 键 词：北五味子 木脂素 正交实验法 超临界二氧化碳流体萃取法 HPLC-APCI—MS/MS 

分 类 号：O657.63[理学—分析化学]