不同生长期油松针叶中倍半萜类化合物分布特征研究  

The Distribution of Sesquiterpenes in Pine Needles During Different Growing Periods

作　　者：何大祥[1,2] 王永莉[1] 李元茂[1,2] 吴应琴[1] 文启彬[1] 杨辉[1] 张虹[1] 王有孝[1] 

机构地区：[1]中国科学院油气资源研究重点实验室,甘肃兰州730000 [2]中国科学院研究生院,北京100049

出　　处：《质谱学报》2011年第5期307-313,共7页Journal of Chinese Mass Spectrometry Society

基　　金：中国科学院战略性先导科技专项课题(XDA05120204);中国科学院知识创新工程重要方向项目(KZCX2-EW-104;KZCX2-YW-Q05-05;KZCX2-YW-104-02);国家重点基础研究发展计划(编号:2005CB422001);国家自然科学基金面上项目(40672123)资助

摘　　要：利用气相色谱-质谱联用技术对现代活体不同生长期(2个月、14个月、26个月)油松松针中提取的有机组分及其分布特征进行了系统分析和研究,检测出丰富的生物标志化合物分子,包括正构烷烃、倍半萜类系列化合物等。通过特征离子碎片和文献对比鉴定,从松针叶片中检测出15种倍半萜类化合物。从生长初期(2个月)至枯萎过程中(14个月、26个月)倍半萜类化合物均有检出并且化合物的种类没有发生变化来看,该类化合物的形成主要是松针叶片在生长过程中的自身产物。随着生长期的增加,松针倍半萜类化合物相对于正构烷烃丰度有明显减少的趋势,而且结果显示,单环芳构化的倍半萜烯类化合物较不饱和二环烯烃倍半萜更易于降解。实验还记录了植物叶子生长过程中环境条件变化的相关信息。The characteristics of biomarkers extracted from needles of the modern living pine with different growing periods were systematically analyzed by GC/MS. Abundant biomar- kers were recognized, such as n-alkanes, sesquiterpenes. 15 sesquiterpenes were detected in the pine needles through the characteristic ion fragmentations. From the initial growth（2 months） to wilting（14 months, 26 months）, all the sesquiterpenes were detected, and the category did not make any change. The result shows that this kind of compounds are formed mainly in the growth of pine needle leaves. With the increase of growing periods, the relative aboundance with the n-alkanesse clearly decreased. And the result shows that sesquiterpenes with one ring aromatizated are much more easily degraded than the unsatu-rated two-ring compounds, these detects are excellent records of the related climate infor- mation.

关 键 词：油松松针 时间梯度 生物标志化合物 倍半萜类化合物 气相色谱-质谱(GC/MS) 

分 类 号：O657.6[理学—分析化学]