抑制水稻隐花色素基因OsCRY1a表达对水稻农艺性状的影响  

Effects of Suppressing OsCRY1a Gene Expression on Rice Agronomic Traits

作　　者：李毓[1,2] 庄伟建[1,3] 王乃元[2] 洪国琴[1] 戴飞 

机构地区：[1]福建农林大学作物科学学院,福建福州350002 [2]福建农林大学作物遗传育种与综合利用教育部重点实验室,福建福州350002 [3]福建省分子与细胞生物学重点实验室,福建福州350002

出　　处：《中国水稻科学》2011年第6期575-579,共5页Chinese Journal of Rice Science

基　　金：福建省科技计划重点资助项目(2008I01021117);国家863计划资助项目(2007AA10Z181)

摘　　要：利用已公布的OsCRY1a基因的序列设计引物,扩增部分基因片段,构建RNAi植物表达载体并转化水稻,导致该基因表达水平下降和功能缺失。根据转基因植株重要农艺性状的表现,分析该基因的功能。结果表明,抑制OsCRY1a基因的表达,水稻开花期推迟16d,株高和粒长明显增加,而其他重要农艺性状如剑叶长、宽和穗长等无明显变化。Using primers designed according to the published sequence of OsCRY1a gene,part of the gene fragment was obtained by PCR and a RNAi expression vector was constructed.The vector was then introduced into rice,leading to the downregulation of the expression level and loss-of-function of the gene.Based on the performance of the transgenic plants,the functions of OsCRY1a was analyzed and deduced.The results indicated that suppressing the expression of OsCRY1a retarded flowering for 16 d with the plant height and grain length significantly increased while other important agronomic traits remained unchanged apparently.

关 键 词：水稻 隐花色素基因 OsCRY1a 农艺性状 基因表达 

分 类 号：S511[农业科学—作物学]