燃煤型氟中毒仔鼠大脑皮质超微结构改变  

Ultrastructure changes in cerebral cortex of offspring rats with coal burning fluorosis

作　　者：桂传枝[1] 冉龙艳[1] 官志忠[1,2] 

机构地区：[1]贵阳医学院附属医院病理科,贵州贵阳550004 [2]贝阳医学院分子生物学重点实验室

出　　处：《中国公共卫生》2011年第11期1367-1369,共3页Chinese Journal of Public Health

基　　金：国家科技部国际合作项目(2010 DFB 30530);贵州省卫生厅优秀医学青年人才科技基金(G2010-20);贵州省高层次人才科研条件特助经费项目(TZJF-2008年-53号);贵州省优秀科技教育人才省长专项基金〔黔省专合字(2009)80号〕

摘　　要：目的复制燃煤型氟中毒仔鼠模型并观察其对大脑皮质超微结构影响。方法 32只SD大鼠随机分为对照组、高氟组;高氟组以地氟病区燃煤烘烤的玉米为主要饲料,复制氟中毒动物模型,饲养6个月后雌雄合笼,取30日龄仔鼠,用氟离子选择电极法检测动物尿、骨及脑氟含量,透射电镜观察动物大脑皮质超微结构改变。结果高氟组仔鼠尿氟(8.52±1.61)mg/L、骨氟(1 874±544)mg/kg、脑氟(0.74±0.26)mg/kg,含量均明显升高,与对照组仔鼠尿氟(0.98±0.26)mg/L、骨氟(1 124±395)mg/kg、脑氟(0.36±0.12)mg/kg比较,差异均有统计学意义(P<0.05或P<0.01);高氟组仔鼠体质量(163±11)g、脑组织重量(1.80±0.10)g、脑器官系数(0.011 1±0.000 4)明显低于对照组仔鼠(147±13)g、(1.54±0.11)g、(0.010 5±0.000 4)(P<0.05或P<0.01);超微结构显示,与对照组仔鼠比较,高氟组仔鼠大脑皮质神经细胞细胞核染色质边集,核膜局部缺如、突触间隙融合、突触结构模糊不清。结论燃煤型氟中毒可以引起仔鼠尿、骨、脑氟含量增高,脑组织重量下降,大脑皮质神经细胞超微结构损伤。Objective To duplicate the animal model with coal burning fluorosis and to observe the influence of fluorosis on the ultrastructure changes of cerebral cortex. Methods Thirty-two SD rats were randomly divided into a control group and a high-fluoride exposed group.The exposed group were fed with the corn polluted in drying processes by burning coal containing high level of fluoride from the endemic fluorosis area to produce the animal model of fluorosis.After six months,the rats mated and their filial generation were observed at age of 30 days and fluoride contents in urine,bone,and brain of the offspring rats were detected by fluorine ion choose electrode.The ultrastructure of cerebral cortex of offspring rats were examined by H-7650 transmission electronic microscope. Results Compared with the offspring rats in the control group,the rats in high-fluoride exposed group had significantly increased fluoride contents in urine（8.52±1.61 vs 0.98±0.26 mg/L）,bone（1874±544 vs 1 124±395 mg/kg）,and brain（0.74±0.26 vs 0.36±0.12 mg/kg）（P0.05 or P0.01）.But the weight of body mass and brain tissue,and the organosomatic index of the brain of the rats of the exposed group were significantly decreased（P0.15 or P0.01）.The nerve cell nuclear envelope local absence,amalgamation of synaptic cleft,and nebulousness of mitochondria in presynaptic membrane and synaptic structure were observed in the high-fluoride exposed rats. Conclusion Chronic fluorosis could be induced in rats feeding with the corn polluted with high level of fluoride and result in increases of urinary fluoride,skeletal fluoride,and fluoride in brain tissue,decreases of the weight of body and brain tissue,and changes of ultrastructure of cerebral cortex in offspring rats with maternal fluoride exposure.

关 键 词：氟化物中毒 仔鼠 大脑皮质 

分 类 号：R595[医药卫生—内科学]