南宁市散发性腹泻诺如病毒分子流行病学分析  

Molecular epidemiology of norovirus infection among sporadic cases of acute gastroenteritis in Nanning city

作　　者：谭冬梅[1] 刘巍[1] 邓丽丽[1] 唐振柱[1] 杨哲[1] 黄兆勇[1] 谢镇国[1] 方志锋[1] 杨进业[1] 董柏青[1] 

机构地区：[1]广西壮族自治区疾病预防控制中心新发传染病研究室,南宁530028

出　　处：《中国公共卫生》2011年第11期1372-1375,共4页Chinese Journal of Public Health

基　　金：广西壮族自治区科学技术厅项目(桂科攻9732027;桂科攻0592007-4;08-05-01D);广西壮族自治区卫生厅科研课题(Z2008214)

摘　　要：目的研究广西南宁市散发性腹泻病例中诺如病毒(norovirus,NV)感染的分子流行病学特征。方法对2007年1月-2008年12月南宁市某医院门诊696例散发腹泻病例进行流行病学资料分析,并应用实时荧光定量逆转录-聚合酶链反应对其粪便标本进行NV及其基因组别检测;随机选择部分阳性标本进行核苷酸序列测定和遗传进化树分析,确定基因型。结果在696份粪便标本中,NV核酸检测阳性183份,总阳性率26.30%,其中180份(98.36%)属于基因II组(genogroup II,GII),另外3份(1.64%)为基因I组(GI);随机选择30株GII组NV进行核苷酸序列分析,绝大多数属于GII.4基因型(26株),其余为GII.12(2株)、GII.7(1株)、GII.16(1株);3株GI组NV分属于GI.2、GI.4、GI.8型;NV所致散发性腹泻在冬春季节呈现高发。NV阳性检出率无性别和年龄组差异。结论NV是南宁市散发性腹泻病例的主要病原体,优势毒株为GII.4型,同时存在多种其他基因型感染。Objective To characterize molecular epidemiological features of norovirus infection among sporadic cases with acute gastroenteritis in Nanning city. Methods The epidemiological data of diarrheic patients from outpatient clinics of a hospital in Nanning city between January 2007 and December 2008 were analyzed.A total of 696 fecal specimens were collected from the patients and screened for the presence of norovirus RNA using real-time reverse transcription-polymerase chain reaction.Some positive specimens were selected randomly and subjected to sequencing and phylogenetic analysis to identify their genotypes. Results Of 696 specimens,183（26.30%） were positive for norovirus RNA;among them,180（98.36%） belong to genogroup II（GII） and the remaining 3（1.64%） belong to genogroup I（GI）.Of the 30 GII strains randomly selected for sequencing analysis,the predominant genotype was GII4（26 cases）,followed by GII12（2 cases）,GII7（1 case）,and GII16（1 case）.The 3 GI strains were classified into three genotypes：GI2,GI4,and GI8.The sporadic norovirus infection occurred preferentially during the cold season.There were no statistical differences in the detection rate among the patients of different genders and age groups. Conclusion The findings suggest that noroviruses are a major etiologic agent of sporadic gastroenteritis in Nanning city.The GII4 is the predominant genotype,co-circulating with other diverse genotypes.

关 键 词：诺如病毒 散发 腹泻 分子流行病学 基因型 

分 类 号：R195[医药卫生—卫生统计学] R373.2[医药卫生—卫生事业管理]