广西肝细胞癌与TGF-β1及IL-12B基因多态性关系  

Polymorphism of TGF-β1 and IL-12B gene and risk of hepatocellular cancer in Guangxi——a case-control study

作　　者：杨艳[1] 仇小强[2] 余红平[1] 曾小云[1] 贝春华[1] 范雪娇[1] 黄金梅[1] 

机构地区：[1]广西医科大学公共卫生学院流行病学教研室,广西南宁530021 [2]桂林医学院

出　　处：《中国公共卫生》2011年第11期1383-1385,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30860247);广西自然科学基金(桂科自0832017Z)

摘　　要：目的探讨广西地区肝细胞癌遗传易感性与人群转化生长因子β1(TGF-β1)基因和白细胞介素12B(IL-12B)基因单核苷酸多态性关系。方法采用以医院为基础的病例对照研究,选取广西户籍的新发肝细胞癌患者608例,选取相同户籍地、年龄、性别和民族频数匹配的非肿瘤患者612例作为对照,采用TaqMan MGB实时荧光定量PCR方法检测TGF-β1 rs1800469(-509 C>T)位点和IL-12B rs3212227(3’UTR+1188A>C)位点基因单核苷酸多态性,比较不同基因型与肝细胞癌患病风险的关系。结果对照组IL-12B基因rs3212227位点的C突变等位基因频率为43.5%,病例组为48.9%,差异有统计学意义(P<0.05);与IL-12B基因rs3212227位点AA基因型相比,携带rs3212227 AC杂合子及携带rs3212227 CC突变基因型的个体发生原发性肝细胞癌的风险明显升高(分别校正OR=1.271,95%CI=0.976～1.655;校正OR=1.515,95%CI=1.092～2.102);IL-12B基因rs3212227位点AC+CC基因型可增加女性和<55岁者罹患肝细胞癌的风险(分别校正OR=2.036,95%CI=1.169-3.548;校正OR=1.529,95%CI=1.129～2.071);肝细胞癌患病风险与TGF-β1基因rs1800469位点多态性无明显关联。结论 IL-12B基因rs3212227位点与广西地区人群原发性肝细胞肝癌发病风险明显关联。Objective To explore the relationship of transforming growth factor beta 1（TGF-β1） gene rs1800469 and interleukin 12B（IL-12B） rs3212227 polymorphisms with the susceptibility to hepatocellular carcinoma（HCC）.Methods A hospital-based case-control study was conducted in 608 cases with HCC and 612 controls matched for age,sex,nationality,and residence in Guangxi Zhuang Autonomous Region.Genotypes of TGF-β1 gene rs1800469（-509 CT）and IL-12B gene rs3212227（3＇UTR＋1188AC） were determined by real-time polymerase chain reaction with TaqMan MGB probe.Results The C allele mutation frequencies of IL-12B rs3212227 in the control group and case group were 43.5% and 48.9%,with a significant difference between the two groups（P0.05）.Compared with wild rs3212227 AA genotype,the IL-12B rs3212227 C allele（adjusted odds ratio=1.336,95% confidence interval：1.040-1.717） was a significant determinant factor for HCC.The IL-12B rs3212227 AC and CC genotype increased the risk of HCC（adjusted OR=1.271,95%CI：0.976-1.655;adjusted OR=1.515,95%CI：1.092-2.102,respectively）.No significant association was observed between the CT genotype and the TT genotype of TGF-β1 rs1800469 compared with the CC genotype（adjusted OR=1.087,95%CI：0.852-1.386;adjusted OR=1.087,95%CI：0.766-1.542,respectively）.The stratified analyses revealed that the AC＋CC genotype of IL-12B rs3212227 significantly increased the risk of HCC among the female and the subjects aged less than 55 years（adjusted OR=2.036,95%CI：1.169-3.548;adjusted OR=1.529,95%CI：1.129-2.071,respectively）.No interaction was observed between TGF-β1 polymorphisms and the risk factors including age,smoking,alcohol drinking,and hepatitis B virus infection for the development of HCC.Conclusion Our results suggest that IL-12B rs3212227 polymorphisms may have independent effects on the susceptibility to HCC in Guangxi population.

关 键 词：肝细胞癌 转化生长因子β1(TGF-β1)基因 白细胞介素12B(IL-12B)基因 单核苷酸多态性 病例对照研究 

分 类 号：Q987[生物学—遗传学]