UVC照射V79细胞后培养液对旁观者细胞影响  

Effects of UVC irradiation to V79 cells cultured in ICM on bystander cells

作　　者：巫生文[1] 靳翠红[1] 刘秋芳[1] 齐鸣[1] 鲁帅[1] 蔡原[1] 

机构地区：[1]中国医科大学公共卫生学院卫生毒理教研室,辽宁沈阳110001

出　　处：《中国公共卫生》2011年第11期1422-1423,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30471475)

摘　　要：目的探讨ultraviolet C诱导的旁观者效应的可能机制。方法用辐射强度为9 mJ/cm2 UVC照射V 79细胞20 s,分别在第4、8、12 h取靶细胞辐照条件培养基(ICM)培养正常细胞24 h。四甲基偶氮唑盐法测定细胞存活率;观察染色体畸变;流式细胞仪检测细胞凋亡率。结果随着ICM时段的往后推移,细胞的存活率逐渐上升,4、8、12 h时段ICM培养的细胞存活率分别为(61.5±3.9)%、(78.8±3.3)%、(84.2±4.1)%,与对照组比较,差异均有统计学意义(P<0.01);染色体畸变率下降,4、8、12 h时段染色体畸变率分别为12.33%、8.67%、5.67%;细胞凋亡率逐渐降低,4、8、12 h时段细胞凋亡率分别为(20.78±2.38)%、(13.45±1.99)%、(10.51±1.53)%,与对照组(3.04±0.39)%比较,差异有统计学意义(P<0.01)。结论用照射剂量为180mJ/cm2 UVC处理靶细胞后的ICM能够诱导出明显旁观者效应,其表现为细胞凋亡。Objective To investigate the mechanism of bystander effect induced by ultraviolet irradiation. Methods The V79 cells were irradiated for 20 seconds with ultraviolet C（UVC） at 9 mJ/cm2 radiation intensity.The irradiated conditioned medium（ICM） was harvested in the phases of 4,8,and 12 hours.Cell viability was evaluated by methyl thiazolyl tetrazolium（MTT） assay.The chromosome aberration was estimated with cell genetics test.Apoptosis rate of V79 cells were determined with flow cytometry（FACs）. Results The cell viability increased with the prologation of harvest time of ICM.The viabilities of 4,8 and 12 hours were 61.5±3.9%,78.8±3.3%,and 84.2±4.1%,which were remarkably different from that of the control（P0.01）.The chromosome aberration rate decreased with the rates of 12.33%,8.67%,and 5.67%.The apoptosis rate descended with the rates of 20.78±2.38%,13.45±1.99%,and 10.51±1.53%,which were noticably higher than that of the control group（P0.01）. Conclusion The ICM harvested form the cells irradiated with 180 mJ/cm2 UVC can induced bystander effect in the form of apoptosis.

关 键 词：UVC 细胞凋亡 旁观者效应 

分 类 号：Q255[生物学—细胞生物学]