女贞子超临界CO_2萃取物遗传和亚急性毒性评价  

Genetic toxicity and subacute toxicity tests of supercritical CO_2 extract of Ligustrum lucidum in rats

作　　者：董茜[1] 单安山[1] 单玉萍[1] 

机构地区：[1]东北农业大学动物科技学院,黑龙江哈尔滨150030

出　　处：《中国公共卫生》2011年第11期1424-1426,共3页Chinese Journal of Public Health

基　　金：国家科技支撑计划项目(2006BAD12B07-11);黑龙江省科技攻关重大项目(GA07B201);东北农业大学创新团队发展计划项目(CXT006)

摘　　要：目的从遗传毒性和亚急性毒性角度对女贞子超临界CO2萃取物进行毒理学评价。方法采用测定小鼠骨髓微核率和大鼠30 d饲养试验对受试动物进行体重、血液学指标、血清生化指标和脏器病理检测。结果在骨髓微核试验中,添加女贞子超临界CO2萃取物各剂量组的骨髓微核率与阴性对照组比较,差异均无统计学意义(P>0.05),但与阳性对照组比较,差异均有统计学意义(P<0.01);在30 d饲养试验中,添加女贞子超临界CO2萃取物的各剂量组动物在给药期均存活,且采食量、体重、行为活动性状等均正常;血液学检测结果,白细胞数9.3×1012～1012/L、红细胞数8.23×109～8.89×109/L、血红蛋白136.4～140.9 g/L和血小板312.3～330.9×109/L,血液生化指标检测结果,谷丙转氨酶41.8～45.8μ/L,谷草转氨酶150.3～153.0μ/L,尿素氮4.8～5.1 mmol/L,肌酐104.5～109.5μmol/L,血糖5.0～5.49 mmol/L,血清白蛋白38.87～40.8 g/L,总蛋白84.3～89.1 g/L,总胆固醇1.4～1.5 mmol/L和甘油三酯0.81～0.87 mmol/L,主要脏器重量和脏器系数均在正常范围内。结论女贞子超临界CO2萃取物不能引起小鼠遗传突变和大鼠亚慢性中毒。Objective To study the safety of supercritical CO2 extract of Ligustrum lucidum with a series of gengtic toxicity and subacute toxicity tests. Methods The genetic toxicity was tested with mouse micronucleus test and subacute toxicity test by 30-day feeding test in rats. Results There was no significant difference in the micronucleus test between low,moderate and high dose treated mice and the negative control group（P0.05）.But compared to that of the positive group,micronucleus rate was significantly different（P0.01）.In the 30-day feeding test,all rats survived with normal feed intake,body weight and behavioral activity.The results of hematological examination were 6.9-10.0×1012/L for white blood cell count,8.23-8.89×109/L for red blood cell count,136.4-140.9 g/L for hemoglobin,and 312.3-330.9×109/L for platelet.The results of biochemical test were 41.8-140.9 μ/L for alanine aminotransferase,50.3-153.0 μ/L for asparate aminotransferase,4.8-5.1 mmol/L for blood urine nitrogen,104.5-109.5 μmol/L for chromium,5.0-5.49 mmol/L for glucose,38.87-40.80 g/L for total protein,1.4-1.5 μmol for total cholesterol,and 0.81-0.87 mmol/L for triglyceride,respectively. The main organ weight and organ coefficient were within the normal range.Pathological examination showed that high-dose group and the control group had no significant difference（P0.05）. Conclusion The results of the study indicate that supercritical CO2 extract of Ligustrum lucidum has no genetic toxicity and subacute toxicity in rats.

关 键 词：女贞子超临界CO2萃取物 微核率 30d饲养试验 

分 类 号：R284.1[医药卫生—中药学]