土鳖虫超微粉体中游离氨基酸的测定及指纹图谱研究  

HPLC fingerprint of free amino acids in micropowder of Eupolyphaga

作　　者：蔡萍[1,2] 万丹[1,2] 肖娟[1,2] 张水寒[1,2] 蔡光先[1,2] 

机构地区：[1]湖南省中医药研究院湖南省中药粉体与创新药物省部共建重点实验室 [2]湖南省教育厅中药粉体技术创新团队,湖南长沙410013

出　　处：《中成药》2011年第10期1645-1648,共4页Chinese Traditional Patent Medicine

基　　金：中医药行业科研专项项目赤芍;马钱子等40种中药粉体行业标准(200807054)

摘　　要：目的建立土鳖虫超微粉体的游离氨基酸的HPLC指纹图谱,考察其主要氨基酸的量。方法采用异硫氰酸苯酯(PITC)柱前衍生,梯度洗脱进行色谱分离,固定相为依利特Hypersil BDS C18柱(4.6 mm×250 mm,5μm),流动相A乙腈-水(4∶1),流动相B醋酸钠缓冲溶液(pH=6.5)-乙腈(97∶3),检测波长为254 nm,体积流量为1 mL/min,柱温为30℃。结果初步建立了土鳖虫超微粉体中游离氨基酸的HPLC指纹图谱,标定了24个共有峰,同时定量分析了6种氨基酸成分。结论建立的方法分离效果好,灵敏、准确、简单,可作为土鳖虫超微粉体质量评价的方法。AIM To determine the HPLC fingerprint of free amino acids in ultramicro powder of Eupolyphaga,and test its contents.METHODS Derived pre-column derivitization with phenyl isothiocyanate（PITC）,Chromatographic conditions were as follows：column Hypersil BDS C18（4.6 mm × 250 mm,5 μm）,mobile phase was（A）acetonitrie-water（4∶ 1）,（B） natrium aceticum buffer solution（pH=6.5）-acetonitrie（97∶ 3）;the detective wavelength was set at 254 nm,column tempemture maintained at 30℃ and the flow rate was 1 mL/min.RESULTSThe fingerprint of micropowder Eupolyphaga was established,and 24 common peaks were found in the fingerprint,and six kinds of amino acids contents were quantitatively analysed.CONCLUSION The method is sensitive,accurate,simple,thereby can be used specifically for the quality control of ultramicro powder of Eupolyphaga.

关 键 词：土鳖虫 超微粉体 指纹图谱 定量测定 柱前衍生化 

分 类 号：R284.1[医药卫生—中药学]