基于Box-Behnken实验优化龙血竭固体脂质纳米粒的制备  

Preparation and optimization of Resina Draconis loading solid lipid nanoparticle by using Box-Behnken design

作　　者：郝吉福[1] 王建筑[1] 郭丰广[1] 孔志峰[1] 李菲[1] 彭新生[2] 

机构地区：[1]山东泰山医学院药学院,山东泰安271016 [2]广东医学院药学院,广东东莞523808

出　　处：《中成药》2011年第10期1713-1718,共6页Chinese Traditional Patent Medicine

基　　金：广东省科技计划(2011B03710058);国家青年科学基金(81102820)

摘　　要：目的基于Box-Behnken实验设计探讨影响龙血竭固体脂质纳米粒的处方因素以优化最佳处方。方法以龙血竭为模型药物,采用乳化蒸发-低温固化法制备固体脂质纳米粒。利用三因素三水平Box-Behnken实验设计,考察单硬脂酸甘油酯的用量(X1),泊洛沙姆188的用量(X2)以及药物的比例(X3)对固体脂质纳米粒包封率(Y1)、浊度(Y2)和载药量(Y3)的影响,通过建立相应的二项式数学模型优化处方。结果最优处方为固体脂质纳米粒中脂质单硬脂酸甘油酯用量为5%,5.39%的poloxmer 188作为乳化剂,含药物量为15%。结论采用Box-Behnken实验设计可用于龙血竭固体脂质纳米粒的处方优化筛选。AIM To apply Box-Behnken design to investigate main and interaction effects of formulation parameters in optimizing solid lipid nanoparticle of Resina Draconis formulation.METHODS Solid lipid nanoparticles were prepared by the emulsion-evaporation and low temperature-solidification technique using Resina Draconis as model drug.A three-factor,three-level Box-Behnken experimental design was employed to construct a second order polynomial equation for the optimized procedure,with the amount of glycerol monostearate（X1）,concentration of poloxamer（X2）,and amount of drug（X3） as the independent variables and the entrapment efficiency（Y1）,turbidity（Y2） and drug loading（Y3） as the response variables.RESULTS The optimization formulation was as follows： the amount of glycerol monostearate was 5%,the concentration of poloxamer was 5.39% and the amount of drug was 15%.CONCLUSION Box-Behnken design demonstrates the available for the optimization of Resina Draconis-loaded solid lipid nanoparticles.

关 键 词：龙血竭 BOX-BEHNKEN设计 固体脂质纳米粒 效应面法 

分 类 号：R944.9[医药卫生—药剂学]