清胆胶囊中5个主要成分的定量测定  

The content determination of five main ingredients in Qingdan Capsule

作　　者：石志娜[1] 周昕[1] 梁晓强[1] 谢瑞芳[1] 张静喆[1] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032

出　　处：《中成药》2011年第10期1722-1726,共5页Chinese Traditional Patent Medicine

基　　金：上海市医学领军人才资助课题(L106048);上海市科委自然科学基金项目(11ZR1436800);国家教育部高等学校博士点基金(200802680005)

摘　　要：目的建立清胆胶囊(大黄、虎杖、陈皮等)中5个主要成分的定量测定方法。方法以虎杖苷、橙皮苷、大黄素、大黄酚、大黄素甲醚为考察指标,采用1100系列安捷伦色谱仪和XDB-C18色谱柱(4.6 mm×250 mm,5μm)及C18保护柱;柱温为25℃;以甲醇-0.1%磷酸水为流动相梯度洗脱,体积流量为1.0 mL/min;检测波长280 nm;检测时间142 min。结果在该色谱条件下,包括上述5个指标成分在内的22个峰得到了良好的基线分离。结论该方法稳定性、准确度和回收率良好。该HPLC方法可用于清胆胶囊的质量控制。AIM To establish the analytical method of quality control for the five effective ingredients in Qingdan Capsule（Rhei Radix et Rhizoma,Polygoni cuspidati Rhizoma et Radix,Citri Reticulatae pericarpium,etc.）.METHODS Polydatin,hesperidin,emodin,chrysophanol,physcion were adopted as the reference substances,HPLC method was established using agilent 1100 HPLC system.A XDB-C18 column（4.6 mm×250 mm,5 μm） coupled with a C18 guard column was used at 25 ℃.A mobile phase consisted of methanol and 0.1%H3PO4 water was used for gradient elution at a flow rate of 1.0 mL/min.Detection wavelength was set at 280 nm,detection time was 142 minutes.RESULTS Under this developed condition,twenty-two peaks were obtained,including above five reference chemical components.CONCLUSION The stability,accuracy and recovery of this method are good.This HPLC fingerprint chromatogram can be used for the quality control of Qingdan Capsule.

关 键 词：清胆胶囊 虎杖苷 橙皮苷 大黄素 大黄酚 大黄素甲醚 

分 类 号：R927.2[医药卫生—药学]