HPLC法测定祖师麻膏药中祖师麻甲素和紫丁香苷  

Determination of daphnetin and syringin in Zushima Plaster by HPLC

作　　者：张晓萍[1] 丁永辉 李玲莉[3] 张妍[1] 倪琳 杨锡 

机构地区：[1]兰州大学药学院,甘肃兰州730000 [2]甘肃省食品药品监督管理局,甘肃兰州730000 [3]甘肃中医学院,甘肃兰州730000 [4]甘肃省食品药品检验所,甘肃兰州730000

出　　处：《中成药》2011年第10期1727-1730,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立定量测定祖师麻膏药中祖师麻甲素和紫丁香苷的高效液相色谱法(HPLC)。方法采用高效液相色谱法,以二极管阵列检测器(PAD)进行测定。色谱柱CAPCELL PAK MG C18(250 mm×4.6 mm,5μm),流动相乙腈-0.4%磷酸(10∶90),体积流量1.0 mL/min,检测波长225 nm,柱温30℃。结果祖师麻甲素、紫丁香苷进样量分别在0.088 8μg～0.266 4μg和0.00 57μg～0.017 1μg范围内呈良好的线性关系(祖师麻甲素r=0.999 6;紫丁香苷r=0.999 3),平均回收率分别为99.95%和98.93%,RSD分别为1.33%和1.76%(n=9)。结论本方法快速,简便,结果准确可靠,重现性好,可用于祖师麻膏药的质量控制。AIM To establish an HPLC method for determining daphnetin and syringin in Zushima Plasters（Barks of root and stem of Daphne giraldii Ntcsche and Daphne tangutica Maxim.） METHODS The HPLC method was conducted with a PAD detecter and the detection wavelength set at at 225 nm.The column was CAPCELL PAK MG C18（4.6 mm×250 mm,5 μm） with mobile phase consisted of acetonitile-0.4%phosphoric acid（10∶ 90） and the flow rate was 1.0 mL/min.The column temperature maintained at 30 ℃.RESULTS The standard curve showed linear in ranges of 0.088 8 μg-0.266 4 μg and 0.005 7 μg-0.017 1 μg for daphnetin and syringin,respectively（daphnetin r=0.999 6 and syringin r=0.999 3）.The average recoveries were 99.95% and 98.93%.The RSD were 1.33% and 1.76%,respectively（n=9）.CONCLUSION This method is quick,simple and accurate with good reproducibility,which can be used for quality control of Zushima Plasters.

关 键 词：祖师麻甲素 紫丁香苷 祖师麻膏药 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]