褶纹冠蚌脏神经节Glu、GABA、DA、Ach和5-HT能神经元的分布  

Distribution of glutamatergic,GABAergic,dopaminergic,acetycholinergic and serotonergic neurons in the visceral ganglion of the bivalve Cristaria plicata

作　　者：肖昱[1] 唐勤[1] 孙妍妍[2] 刘玥[2] 李葆明[1,2] 雷光春[3] 吴小平[1,3] 

机构地区：[1]南昌大学生命科学研究院,江西南昌330031 [2]复旦大学神经生物学研究所,上海200032 [3]北京林业大学自然保护区学院,北京100083

出　　处：《海洋科学》2011年第10期46-51,共6页Marine Sciences

基　　金：国家自然科学基金资助项目(30860045);江西省主要学科学术和技术带头人资助项目(2003-154;2009DD01100)

摘　　要：运用H.E.染色法和SP免疫组织化学技术,观察了褶纹冠蚌(Cristaria plicata(Leach)中枢神经系统脏神经节的谷氨酸(glutamate,Glu)、γ-氨基丁酸(γ-aminobutyric acid,GABA)、多巴胺(dopamine,DA)、乙酰胆碱(acetylcholine,Ach)和5-羟色胺(5-hydroxytryptamine,5-HT)神经元的分布和形态。Glu神经元、GABA神经元和DA神经元多为小型细胞,Ach神经元多为中型细胞,5-HT神经元多为大、中型细胞,偶见小型细胞。并且DA神经元分布在脏神经节的整个胞体区,Ach神经元多分布于近外套神经和鳃神经根部,而在中央纤维网区域存在大量GABA能神经纤维。结果表明,褶纹冠蚌脏神经节内含有Glu、GABA、DA、Ach和5-HT能神经元,但这5种神经元的形态和分布有所不同。The distribution and morphology of glutamate-, GABA-, dopamine- acetycholine- and sero- tone-containing neurons in the visceral ganglion of the bivalve Cristaria Plicata were observed by Hematoxy- lin-Eosin staining method and SP immunohistochemical technique. The cellular layer of the visceral ganglion had glutamatergic, GABAergic and dopaminergic neurons in small size and acetycholinergic neurons in medium size, and most of serotonergic neurons were in large cell bodies. Moreover, dopaminergic neurons were widely distributed in the whole cortex of the ganglion, whereas acetycholinergic neurons were mainly localized near the pallial nerves and the roots of branchial nerves. GABAergic fibers could be seen in the central neuropil. The present results indicate that the visceral ganglion in the visceral ganglion of the Cristaria plicata contains glutamatergic, GABAergic, dopaminergic, aeetycholinergic and serotonergic neurons with different quantities and densities.

关 键 词：褶纹冠蚌(Cristaria plicata(Leach) 脏神经节 神经递质 神经元 

分 类 号：Q954.2[生物学—动物学]