温度和盐度对马氏珠母贝精子激活率及活力的影响  

Effect of temperature and salinity on the sperm activation and motility in Pinctada fucata martensii (Dunker)

作　　者：王辉[1] 罗明明[1] 朱晓闻[1] 吕文刚[1] 李艳红[1] 王亚男[1] 刘志刚[1] 

机构地区：[1]广东海洋大学水产学院,广东湛江524025

出　　处：《海洋科学》2011年第10期57-63,共7页Marine Sciences

基　　金：广东省高校热带海产无脊椎动物养殖工程研究中心科研项目(GCZX-A0909);国家科技支撑计划项目(2007BAD29B01);农业部公益性行业(农业)科研专项子课题(nyhyzx07-047);广东省重大科技兴海(兴渔)项目(A200708C01)

摘　　要：采用中心组合试验设计(CCD)和响应曲面法,在实验室条件下研究温度(T)、盐度(S)对马氏珠母贝(Pinctada martensii)精子激活率及活力的影响并建立了模型。实验设置的温度范围为15～45℃,盐度范围为12～48。结果表明,马氏珠母贝精子激活率与活力随着温度、盐度的升高呈先升高后降低的趋势;盐度的一次效应和二次效应以及温度的二次效应对精子激活及活力的影响极显著(P<0.01),温度和温盐的互作效应对精子激活率及活力的影响不显著(P>0.05);保持马氏珠母贝精子激活率较高时的温度为26～31℃,盐度为25～30;保持马氏珠母贝精子活力较大时的温度为24～35℃,盐度为22～30;实验建立了温度和盐度对马氏珠母贝精子激活率及活力影响的模型,通过模型同时优化得出温度30.04℃,盐度为26.86时,马氏珠母贝精子激活率最高、活力最大,分别为85.51%和57.82%。Central composite circumscribed experimental design and response surface analysis method was employed to study effects of different combinations of temperature and salinity on the sperm activation and relative motility of Pinctada fucata martensii （Dunker）, and the equation of temperature, salinity and sperm activation rate and motility was established. The temperature was raning from 15 to 45℃, and the salinity 12 to 48. The sperm activation rate and motility were first increased and then decreased as temperature and salinity increased; The sperm activation rate and motility were significantly affected by the linear and quadratic effects of salinity and quadratic effects of temperature （P〈0.01）, but The synergistic effect between salinity and temperature was not significant （P〉0.05）; the suitable temperature and salinity of sperm activation rate were 26-31℃ and 25-30, respectively, and of sperm motility were 24-35℃ and 22-30, respectively. By means of optimization technique, the optimal point was found at temperature 30.04℃ and salinity 26.86. At this optimum point, maximumal sperm activation rate and sperm motility were 85.51% and 57.82%, respectively, with the desirability as high as 97%.

关 键 词：马氏珠母贝(Pinctada martensii) 精子激活 活力 响应曲面 优化 

分 类 号：Q28[生物学—细胞生物学]