沙眼衣原体临床株基因分型及体外药敏试验结果分析  

Genotyping of Chlamydia trachomatis clinical isolates and analysis of drug sensitive test in vitro

作　　者：杨丽娜[1] 江勇[1] 刘全忠[2] 

机构地区：[1]天津医科大学第二医院,天津300211 [2]天津医科大学总医院

出　　处：《山东医药》2011年第39期1-3,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30872285)

摘　　要：目的分析沙眼衣原体(Ct)临床株的基因型分布及其对常用抗菌药物的敏感性;进一步提高泌尿生殖道Ct感染的治疗水平。方法采用聚合酶链反应和限制性内切酶片段长度多态性分析方法对52株Ct行基因分型;测定9种抗菌药物对其的最低抑菌浓度(MIC)。结果 52株Ct中E型49株、G型2株、J型1株。9种抗菌药物对Ct的MIC以红霉素最高,克拉霉素最低;共发现13株红霉素耐药株,未发现对其他药物的耐药株。结论天津地区Ct感染以E型为主,对克拉霉素较为敏感;已发现对红霉素耐药的Ct,应引起临床注意。Objective To assess the genotyping distribution of Chlamydia trachomatis（Ct） clinical isolates and its susceptibility to common antimicrobial agents;to improve the treatment level of urogenitaltract infection.Methods Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP）was performed to determine the genotype of 52 Ct;the MICs of 9 antimicrobial agents to Ct were determined.Results There were 49 strains of E type,2 strains of G type,and 1 strain of J type.The highest MIC of 9 antimicrobial agents to Ct was erythromycin,and the lowest was clarithromycin;13 strains were found resistant.Conclusions The most prevalent genotypes of Ct infection in Tianjin is E type,which is susceptive to clarithromycin;the clinic should take attention to that Ct strains resistant to erythromycin have been found.

关 键 词：沙眼衣原体 基因型 药敏试验 

分 类 号：R691.3[医药卫生—泌尿科学]