广州市0～14岁儿童卡介苗特异性Th1细胞免疫水平分析  

Analysis of BCG-specific Th1 cellular immune response in children aged from 0 to14 years old in Guangzhou

作　　者：乔丹[1] 陈慎 吴长有[1] 王慧[1] 李丽[1] 

机构地区：[1]中山大学中山医学院免疫学研究所热带病防治研究教育部重点实验室,广州510080 [2]珠海市第二人民医院检验科,519020

出　　处：《免疫学杂志》2011年第11期979-982,986,共5页Immunological Journal

基　　金：中央高校基本科研业务费专项资金(09YKPY76)

摘　　要：目的分析广州市0～14岁儿童卡介苗特异性Th1细胞免疫水平。方法随机抽取288例广州市妇女儿童医疗中心儿童院区健康体检的0～14岁儿童静脉抗凝血,经BCG刺激后,用酶联免疫吸附试验(ELISA)检测Th1细胞因子(IFN-γ,TNF-α和IL-2)的水平,并用流式细胞术(FACS)检测IFN-γ和TNF-α的细胞来源。结果 BCG刺激可显著诱导儿童外周血细胞产生IFN-γ(median=726.37 pg/ml),TNF-α(median=934.98 pg/ml)和IL-2(median=48.25 pg/ml)。与不刺激组相比,差别具有统计学意义(n=288,P<0.001)。此外,IFN-γ的产生水平与年龄呈一定的正相关(r2=0.4069,P<0.05)。流式分析结果表明,BCG刺激可诱导CD4+T细胞和非CD4CD8细胞分泌IFN-γ或TNF-α,而不诱导CD8+T细胞产生细胞因子。经BCG刺激后,分别有35.71%和39.29%的儿童CD4+T细胞能够产生IFN-γ和TNF-α。结论广州市0～14岁儿童外周血细胞经BCG刺激后,主要是由CD4+T细胞和非CD4CD8细胞产生Th1细胞因子,但是CD4+T细胞能够产生Th1细胞因子的儿童比例较低,这可能是卡介苗接种免疫效果并不理想的原因之一。This study is aimed to investigate BCG-specific Th1 cellular immune responses in children aged from 0 to14 years old in Guangzhou.We randomly recruited 288 cases of healthy check-up children from the Children＇s Hospital of Guangzhou.Th1 cytokines（IFN-γ,TNF-α,and IL-2） were detected by ELISA following stimulation with BCG.The production of IFN-γ and TNF-α following BCG stimulation were further analyzed by FACS.Significantly higher levels of IFN-γ（median = 726.37 pg/ml）,TNF-α（median = 934.98 pg/ml） and IL-2（median = 48.25 pg/ml） were detected following BCG stimulation as compared with the unstimulated condition（n = 288,P 0.001）.And IFN-γ level was positively related with the age of the children（r2 = 0.4069,P 0.05）.In addition,IFN-γ and TNF-α were mainly produced by CD4＋ T cells but not CD8＋ T cells,after BCG stimulation.IFN-γ was also produced by nonCD4CD8 cells.In conclusion,only 35.71% to 39.29% of children are demonstrated to produce IFN-γ and TNF-α by CD4＋ T cells following stimulation with BCG,which might be the possible reason for the limited protective effect of BCG vaccination.

关 键 词：卡介苗 细胞因子 酶联免疫吸附试验 流式细胞术 

分 类 号：R392[医药卫生—免疫学]