乔木茵芋中黄酮和生物碱类化合物及其镇痛活性研究  

Studies on flavonoid and alkaloid from Skimmia arborescens Anders. ex Gamble and its pain releasing effect

作　　者：何雷[1,2] 杨顺丽 吴德松 字刚 崔涛[1,2] 丁中涛[2] 

机构地区：[1]云南省药物研究所天然药物筛选研究中心,云南昆明650111 [2]云南大学化学科学与工程学院,云南昆明650091

出　　处：《云南大学学报（自然科学版）》2011年第6期705-709,共5页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省科研院所技术开发研究专项(2004KFZX-12)资助

摘　　要：对乔木茵芋进行化学成分研究.采用硅胶柱色谱、葡聚糖凝胶LH-20柱色谱、RP-C18柱色谱等方法分离化学成分,1H NMR、13C NMR等方法进行结构鉴定,从中分离鉴定了5个黄酮类化合物:槲皮素(1),槲皮苷(2),山奈酚-3,7-葡萄糖苷(3),枸橘苷(4),橙皮苷(5);1个生物碱类化合物:1-甲基-2-苯基-4-喹啉酮(6).所有化合物均为首次从该种植物中分离得到.并采用小鼠醋酸扭体法和热板法检测乔木茵芋总提取物及乔木茵芋总生物碱提取物的镇痛活性.结果显示乔木茵芋总提物和乔木茵芋总生物碱提取物均可明显抑制醋酸所致小鼠扭体反应,此外乔木茵芋总生物碱提取物还可明显提高小鼠对热刺激的痛阈值,具有较好的镇痛活性.To study the chemical constituents of Skimmia arborescens. The chemical constituents were isolated by silica gel column chromatography, Pharmadex LH - 20, RP - C18, and the ^1H NMR, ^13 C NMR spectroscopic analysis were employed for the structural elucidation. Six Compounds were isolated from Skimmia arborescens. Their structures were elucidated as quercetin （ 1 ）, quercetin 3 - O - α - L - Rha （ 2 ）, kaempferol - 3,7 - o -β - D - glucosides （ 3 ）, poncirin （4）, hesperidin （ 5 ）, 1 - methyl - 2 [ （Z） - 7 - phenyl ] - 4 （ 1 H ） - quinolone （ 6 ）, The 6 compounds were isolated from the plant for the first time. Using acetic acid writhing and hot plate test in mice, the pain releasing effects of the total extract and the total alkaloids from the plant were evaluated. The results indicated that both the total extract and total alkaloids of Skimmia arborescens could markedly decrease mice writhing caused by acetic acid. Furthermore, the total alkaloid of Skimmia arborescens could extend evidently pain threshold in mice.

关 键 词：乔木茵芋 黄酮 生物碱 镇痛活性 

分 类 号：Q949.752.7[生物学—植物学] R284[医药卫生—中药学]