丹芍化纤胶囊对大鼠肺纤维化内质网应激相关蛋白GRP78及NF-κB表达的影响  

The effect of Dan Shao Hua Xian Capsule on expression of endoplasmic reticulum stress related protein GRP78 and NF-κB in bleomycin-induced pulmonary fibrosis

作　　者：韩冰[1] 谢汝佳[1] 张成俊[1] 何小飞[1] 杨婷[1] 杨勤[1] 

机构地区：[1]贵阳医学院病理生理学教研室,贵阳550004

出　　处：《中华中医药杂志》2011年第11期2551-2554,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：贵州省科技攻关项目(No.黔科合SZ字[2008]3049号);贵州省科技厅国际科技合作项目(No.黔科合外G字[2010]7016号)~~

摘　　要：目的:观察内质网应激相关蛋白葡萄糖调节蛋白78(GRP78)及核因子-κB(NF-κB)在盐酸博来霉素诱导的肺纤维化中的变化及中药丹芍化纤胶囊对其表达的影响。方法:选取SD大鼠30只,随机分为正常组、肺纤维化模型组和丹芍化纤胶囊治疗组,每组各10只。正常组气管内滴注生理盐水,模型组和丹芍组气管内一次性灌注盐酸博来霉素5mg/kg诱导肺纤维化,丹芍组于造模第2天给予丹芍化纤胶囊混悬液灌胃(0.8g.kg-1.d-1)治疗,正常组及模型组则给予等量生理盐水灌胃。实验第28天称大鼠体质量,处死各组大鼠,称肺湿重并收集肺组织,行HE和Masson染色观察肺组织病理变化;采用免疫组织化学的方法检测肺组织中GRP78及NF-κB蛋白的表达情况。结果:与正常组比较,模型组大鼠肺组织中GRP78及NF-κB表达明显升高(P<0.01);经丹芍化纤治疗后,大鼠肺组织中GRP78及NF-κB蛋白表达显著降低(P<0.01)。结论:GRP78及NF-κB可能参与了盐酸博来霉素诱导的肺纤维化过程。丹芍化纤胶囊对肺纤维化的干预机制可能与抑制肺组织中GRP78及NF-κB的表达,减轻内质网应激所引起的损伤有关。Objective：To investigate the effect of Dan Shao Hua Xian Capsule on expression of endoplasmic reticulum stress related protein GRP78 and NF-κB in bleomycin-induced pulmonary fibrosis.Methods：Thirty SD rats were randomly divided into normal control group,pulmonary fibrosis model group and DSHX Capsule therapeutic group.Pulmonary fibrosis model was induced by intratracheal instillation of single dose bleomycin 5mg/kg.DSHX Capsule 0.8g·kg-1·d-1 was administered daily by oral in the therapeutic groups at the second day after bleomycin.All rats were killed on the 28th day.Detected the level of GRP78 and NF-κB protein in pulmonary tissue by immunohistochemistry.Results： Compared with the control group,the levels of GRP78 and NF-κB in model group were increased（P0.01）,after the treatment of DSHX Capsule,the levels of GRP78 and NF-κB were decreased（P0.01）.Conclusion： GRP78 and NF-κB play an important role in the pulmonary fibrosis.DSHC Capsule could reduce pulmonary fibrosis induced by bleomycin,which mechanism may be to decrease the expression of GRP78 and NF-κB and alleviate the damage result from endoplasmic reticulum stress.

关 键 词：肺纤维化 丹芍化纤胶囊 内质网应激 葡萄糖调节蛋白78 核因子-κB 

分 类 号：R285.5[医药卫生—中药学]