C肽检测系统评价及临床应用  

Evaluation and clinical application in detection of serum C-peptide

作　　者：余清[1] 滕凤猛[1] 梁鑫[1] 陈秋霞[1] 

机构地区：[1]江苏省中医院,210029

出　　处：《实用医技杂志》2011年第11期1142-1143,共2页Journal of Practical Medical Techniques

摘　　要：目的对化学发光微粒子免疫检测法检测c肽的方法进行评估和初步应用。方法连续5d测定同一标本每次重复3次。计算重复性、实验室内精密度、真实度、功能灵敏度和线性范嗣；用美国临床实验室标准化委员会（EP15-A）提供的方案对该方法的重复性、实验室内精密度、功能灵敏度和线性范围进行研究。结果该方法的功能灵敏度为0．60ng／mL；性范围0-18．30ng／mL；~部精密度为7．00％-12．29％，总不精密度为6．63％-8．35％。结论化学发光微粒子免疫检测系统检测C肽各项性能指标良好，符合要求，系统推荐用肝素抗凝管。Objective To evaluate the determination method of C-peptide which is detected by chemiluminesent micropaticle immunoassay. Methods A series of different concentration lever serum were analyzed by 5 days and repeated three times. The functional sensitivity, linearity, imprecision were studied according to National Committee for Clinical Laboratory Standards （NCCLS） EP-15A protocol. Results The functional sensitivity of this method was 0.6 ng/mL, the linearity range was 0-18.30 ng/mL, within-run and total imprecision were 7.00%-12.29% and 6.63%-8.35% respectively. Conclusion The method used to detect the C peptide level has good performance and corresponds to clinical needs. Heparin plasma is recommended for detection.

关 键 词：C肽 化学发光测定法 评价研究 

分 类 号：R446.6[医药卫生—诊断学]