siRNA靶向沉默integrin β4对人支气管上皮细胞TLR4基因表达的影响  

Influence of Integrin β4 siRNA on TLR4 Gene Expression in HBECs

作　　者：常永丽[1,2] 秦晓群[1] 向阳[1] 刘惠君[1] 朱晓琳[1] 刘持[1] 

机构地区：[1]中南大学湘雅医学院,410078 [2]长治医学院生理学教研室

出　　处：《长治医学院学报》2011年第5期321-323,共3页Journal of Changzhi Medical College

基　　金：国家自然科学基金项目(编号81170024)

摘　　要：目的:观察siRNA(small interfering RNA)靶向沉默integrinβ4对人支气管上皮细胞(human bronchial epithelial cells,HBECs)TLR4(toll样受体4)基因表达的影响。方法:化学合成inte-grinβ4 siRNA片段,经脂质体转染人支气管上皮细胞株,采用Western-blotting验证沉默效率,细胞化学技术(ICC)观察HBECs经OVA(卵白蛋白)刺激后TLR4基因表达的改变。结果:siRNA转染48 h可显著抑制HBECs中integrinβ4的蛋白表达;与对照组相比,经siRNA转染的人支气管上皮细胞株在OVA刺激1 h后,TLR4表达明显上调。结论:siRNA可靶向、高效沉默人支气管上皮细胞株integrinβ4,显著上调OVA刺激后TLR4的表达。Objective：To study the influence of integrin β4 small interfering RNA on toll like receptor 4（TLR4）gene in human bronchial epithelial cells（HBECs）.Methods：The specific integrin β4 siRNA was chemical synthesis and transfected into HBECs by lipofectamine.The integrin β4 protein abundance was determined by western blotting,immunochemistry was used to deteched the changes of TLR4 gene expression in OVA stimulated HBECs.Results：Integrin β4 protein was decreased obviously after siRNA transfected for 48 h in HBECs.The expression of TLR4 up-regulated in siRNA transfected HBECs stimulated by OVA for 1 h compared to negtive control.Conclusion： The siRNA can effectively down-regulate integrin β4,which Also improved the TLR4 gene expression in OVA stimulated HBECs.

关 键 词：SIRNA TLR4 人支气管上皮细胞 

分 类 号：R332.2[医药卫生—人体生理学]