一株耐碳酸盐Bacillus属细菌的鉴定与特性分析  

Analysis of Charactistics and Identification of a Carbonateresistant Bacillus sp.

作　　者：石伟 高野哲夫[2] 柳参奎[1] 

机构地区：[1]东北油田盐碱植被恢复与重建教育部重点实验室,东北林业大学盐碱地生物资源环境研究中心,哈尔滨150040 [2]东京大学亚洲生物资源环境研究中心,东京1880002

出　　处：《基因组学与应用生物学》2011年第5期603-607,共5页Genomics and Applied Biology

基　　金：国家林业局948项目(2008-4-29);黑龙江省杰出青年科学基金(JC200609)共同资助

摘　　要：本研究从松嫩平原pH值在10.0以上、植物难以生存的斑块状裸地中分离到一株细菌SA-5,经鉴定该菌株为革兰氏染色阴性,杆状,接触酶阳性,氧化酶阴性,菌落呈橙黄色的好氧细菌,可水解淀粉。抗逆特性分析显示,菌株SA-5可在高达275mmol/L Na2CO3或pH 12.0以及2.0mol/L NaCl培养基中生长,中性条件下生长明显受到抑制,其中对碳酸盐的耐受性远高于普通植物。根据16SrDNA序列等分析结果显示,菌株SA-5属于Bacillus属,仅与菌株Bacillus aurantiacus K1-5T相似性最高,为99%,与其余已发表菌株均低于97%。由以上结果可见,菌株SA-5是Bacillus属中一株嗜碱耐盐菌,并且菌株SA-5对耐碳酸盐基因的开发具有潜在的价值。A strain SA-5 was isolated from an extreme soda saline-alkali soil in Songnen Plain,in which the plants are difficult to survive in bare land with pH greater than 10.5.Strain SA-5 was Gram stain negative,rod-shaped catalase-positive,oxidase-negative and formed orange colonies,which was hydrolyzed in starch.Stress resistance analysis showed that,SA-5 strains in 275 mmol/L Na2CO3 or pH 12.0 or 2.0 mol/L NaCl medium could grow normally.The strains were restrained from neutral condition,which could tolerate carbonate much more than the common plants.Based on the analysis results such as 16S rDNA gene sequence comparisons,SA-5 was belonged to genus Bacillus,and it had the highest similarity 99% to Bacillus aurantiacus K1-5T,lower than 97% with other published lines.The above results showed that SA-5 strains was obligate alkaliphilic and halotolerant bacteria,and SA-5 strains on the development of carbonate-resistance gene had the potential value.

关 键 词：16S RDNA序列 芽孢杆菌属 嗜碱耐盐菌 

分 类 号：Q93[生物学—微生物学]