高效液相色谱法测定人血浆中氟西汀浓度  

Determination of the concentration of Fluoxetine in human plasma by HPLC

作　　者：刘文宪[1] 陈清霞[2] 刘伟忠[1] 温预关[1] 王华成[1] 

机构地区：[1]广州市脑科医院国家药品临床研究基地,广州510370 [2]中山大学附属第二医院药学部,广州510120

出　　处：《海峡药学》2011年第10期207-209,共3页Strait Pharmaceutical Journal

基　　金：广东省自然科学基金资助项目(编号:8151037001000001)

摘　　要：目的建立测定人血浆中氟西汀浓度的高效液相色谱法。方法以DiamonsilTMC18反相柱（150mm×4．6mm，5μm）为色谱柱，流动相为0．03mol·L-1醋酸铵．甲醇（27：73）；流速：0．8mL·min-1；柱温：40℃；检测波长：260nm。以乙酸乙酯与二氯甲烷（80：20）为提取剂。结果氟西汀的低、中、高（10．0。40．0。320．0ng·mL-1）3种浓度平均回收率分别为101．56％，98．61％。97．92％。提取回收率分别为71．12％，73．71％，76．59％；日内、日间差RSD均低于9％（n=5）；分析方法的检测限为5．0ng·mL-1；线性范围为10．0--320．0ng·mL-1。线性方程：Y=98．12X＋1．71．r=0．9989（n=8）。结论该方法灵敏、准确、简单、快速，可用于氟西汀临床血药浓度监测和药动学研究。OBJECTIVE To establish a method for determining the concentration of fluoxetine in human plasma by HPLC. METHODS The drug from plasma was analyzed in a reverse phase HPLC system CI8 column （150mm×4.6mm,5μm） ;mobile phase consisted of 0.03mol·L-1 ammonioum-methanol （27 ： 73） ; the flow rate was 0.8mL·min-1 ; the detection wavelength was at 260nm. Ethyl acetate and dichloromethane（80：20）was used as extracting solvent. RESULTS The average recoveries of fluoxetine in low, middle and high concentrations （10.0, 40.0,320.0ng·mL-1 ） were 101.56 %, 98.61% and 97.92 %, respectively. The intra-day and inter-day variation（RSD） was less than 9 % （n = 5）. The calibration curve of fluoxetine showed good linearity, r = 0. 9989 （n = 8）, over the range of 10.0-- 320.0ng·mL-1. The regression equation was Y = 98.12X ＋ 1.71, r = 0. 9989 （n = 8）. The minimum detectable concentration of fluoxetine was 5.0ng·mL-1. CONCLUSION The method is sensitive, accurate, simple and quick, which can be used for clinical drug monitoring and pharmacokinetics studies of fluoxetine.

关 键 词：氟西汀 血药浓度 高效液相色谱法 

分 类 号：R969.1[医药卫生—药理学]