应用细胞膜片复合硅胶管内支撑构建组织工程管状软骨  

Construction of Scaffold-Free Cylindrical Cartilage Combined with Cell Sheet and Silicon Tube

作　　者：王健[1] 刘霞[1] 肖苒[1] 曹谊林[1] 

机构地区：[1]北京协和医学院中国医学科学院整形外科医院研究中心,北京市100144

出　　处：《组织工程与重建外科杂志》2011年第5期241-243,共3页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：北京市科委科技计划重大项目资助(D090800046609003);卫生部部属医院临床学科重点项目;国家自然科学基金青年科学基金项目(30801192);北京市自然科学基金(7102134);高等学校博士学科点专项科研基金(新教师基金课题;200800231078)

摘　　要：目的研究利用羊耳软骨细胞形成细胞膜片，构建无细胞支架组织工程管状软骨的可行性。方法体外培养扩增羊耳软骨细胞至第2代，以2．0×10^6cells／mL的高密度，培养7d，形成直径为35mm的圆形细胞膜片，将细胞膜片均匀包裹于硅胶管，植入裸鼠背部皮下，4周后取材检测。以大体观察、组织学、免疫组织化学等方法，对形成的软骨进行评价。结果大体观察可见形成良好的管状软骨，HE染色见软骨陷窝形态规则，番红0染色及Ⅱ型胶原免疫组化均呈阳性表达。结论细胞膜片复合硅胶管内支撑的方法能形成管状软骨，为气管软骨的再造提供了新的方法。Objective To explore the feasibility of fabricating a scaffold-free cylindrical cartilage using cell sheet with silicon tube as interior support in vivo. Methods Auricular chondrocytes were harvested from goat and the second passage of auricular chondrocytes were cultivated with high-density of 2.0~106 cells/mL to form a chondrocyte sheet with the diameter of 35 ram. Then the silicon tube covered with cell sheet was implanted into the back of nude mice. The tissue engineered cylindrical cartilages were evaluated by gross observation, histological and immunohistochemical examination. Results The cartilage was sufficiently elastic and stiff to maintain the structure without disruption. Cartilage tissue was observed by HE staining, Safranin O staining and immunohistochemical staining for type ]1 collagen. Conclusion A novel technique was developed for fabricating engineered cylindrical cartilage using cell sheet with silicon tube in vivo.

关 键 词：无细胞支架 细胞膜片 管状软骨 气管软骨 

分 类 号：Q813.12[生物学—生物工程]