低氧环境和血清饥饿对内皮祖细胞死亡率的影响  

Effects of Low Oxygen and Serum Deprivation on Death Rate of Endothelial Progenitor Cells

作　　者：连锋[1] 薛松[1] 顾萍 张谷兰[1] 吴学军[1] 朱洪生[1] 

机构地区：[1]上海交通大学医学院附属仁济医院心胸外科,上海市200127 [2]上海儿童医学中心实验诊断中心,上海市200127

出　　处：《组织工程与重建外科杂志》2011年第5期254-257,共4页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：国家自然科学基金(30170930);上海市自然科学基金(0441 19715;08ZR1413600)

摘　　要：目的探讨低氧环境和血清饥饿对骨髓来源内皮祖细胞死亡率的影响。方法取SD大鼠骨髓,分离出内皮祖细胞,并用免疫荧光法鉴定。取第2代细胞,分别在常氧环境(21%O_2)合并正常血清(20%浓度)或血清饥饿(0%、2%浓度)条件下培养48 h,或是低氧环境(3%O_2)合并正常血清或血清饥饿条件下培养48 h、72 h、96 h、120 h。用Live/Dead染色,结合图像分析,计算细胞死亡率。结果短期处于低氧环境中,内皮祖细胞的死亡率无明显变化(p>0.05)。在血清饥饿条件下,细胞死亡率显著升高(p<0.01)。如果长期处于低氧环境中,与正常血清培养相比,血清饥饿培养时细胞死亡率显著升高(p<0.01),72 h时达(96.30±3.18)%,120 h时达100%。结论缺氧环境和血清饥饿对内皮祖细胞的存活均有不良影响,其中血清饥饿的影响更大。Objective To explore the effects of low oxygen and serum deprivation （SD） on death rate of bone marrowderived Endothelial Progenitor Ceils （EPCs）. Methods EPCs were isolated from bone marrow of Sprague-Dawley （SD） rats and identified by immunofluorescence assay. Passage 2 EPCs were exposed to normal oxygen （21% 02） and 0%, 2%, or 20% fetus bovine serum （FBS） for 48 hours, or low oxygen （3% 02） and 0%, 2%, or 20% FBS for 48, 72, 96, and 120 hours. Cell death rate was calculated by Live/Dead staining and image analysis. Results Cell death rate was not affected by low oxygen for 48 hours （P〉0.05）, but affected greatly by serum deprivation （P〈0.01）. In a low-oxygen environment for longer hours, cells exposed to serum deprivation （0%, 2% FBS） showed much higher death rate, as compared with ceils exposed to 20% FBS （P〈0.01）. The death rate reached （96.30±3.18）% for 72 hours and 100% for 120 hours. Conclusion Survival of EPCs is affected by both low oxygen and serum deprivation, of which serum deprivation plays a more dominant role.

关 键 词：内皮祖细胞 血清饥饿 缺氧 死亡率 

分 类 号：Q813.11[生物学—生物工程]