骨科分离80株金黄色葡萄球菌的mecA基因检测及耐药性分析  

Detection of mecA gene in 80 strains of Staphylococcus aureus isolated from orthopedics department and drug resistance

作　　者：陈旭宏[1] 鲍丰[1] 吴胜军[2] 陈继营[3] 

机构地区：[1]温州医学院附属义乌医院骨科,浙江义乌322000 [2]浙江大学医学院附属邵逸夫医院检验科,浙江杭州310016 [3]解放军总医院关节外科,北京100853

出　　处：《中华医院感染学杂志》2011年第21期4438-4440,共3页Chinese Journal of Nosocomiology

摘　　要：目的分析骨科分离的80株金黄色葡萄球菌耐药性,检测金黄色葡萄球菌的mecA基因,探讨金黄色葡萄球菌的耐药机制,指导临床合理应用抗菌药物。方法对2007年1月-2010年12月骨科患者分离的80株金黄色葡萄球菌进行耐药性分析,采用PCR检测金黄色葡萄球菌的mecA基因。结果检出的80株金黄色葡萄球菌中,体外药物敏感性试验发现,均对万古霉素、利奈唑胺敏感率为100.0%,而对青霉素、红霉素等敏感性较低,其中有46株耐甲氧西林金黄色葡萄球菌(MRSA),阳性率达到57.5%,采用PCR方法检测发现46株MRSA携带mecA基因,而对34株甲氧西林敏感金黄色葡萄球菌(MSSA)检测,发现有5株携带mecA基因。结论骨科MRSA分离率较高,而且均具有较高的耐药性,并且mecA基因PCR检测阳性与耐头孢西丁金黄色葡萄球菌的临床意义基本一致。OBJECTIVE To investigate the resistant mechanism of Staphylococcus aureus and study the reasonable use of antibiotic by analyzing the resistant rate of eighty S. aureus strains, which were separated from orthopedics department, and also detect the mecA gene of S. aureus. METHODS We retrospectively analyzed resistance of eighty strains S. aureus separated from orthopedics department in our hospital during Jan 2007 to Dec 2010. We detected the mecA gene by PCR. RESULTS Using the vitro sensitivity test, we found that there were all sensitive to Vancomycin and Linezolid, but resistant to Penicillin and Erythomycin. There were forty-six strains MRSA, the positive rate was 57.50%. Using the PCR method, we found that all the forty-six strains MRSA contain the mecA gene and five MSSA strains contain mecA gene of the thirty-seven strains MSSA. CONCLUSION The separating rate is high in orthopedics department of our hospital, all of them were more resistant to all kinds antibiotics. We also find that cefoXition disk diffusion method can be used reliably in detecting and confirming MRSA.

关 键 词：耐甲氧西林金黄色葡萄球菌 MECA基因 体外药敏试验 骨科 

分 类 号：R378.11[医药卫生—病原生物学]