高效液相色谱法测定白饭树中槲皮素的含量  

Determination of Quercetin in Flueggea virosa (Roxb. ex Willd.) Baill

作　　者：夏爱军[1] 韦少宣[2] 廖厚知 梁园[1] 张云波[1] 

机构地区：[1]解放军303医院药剂科,广西南宁530021 [2]广西中医学院药学院中药教研室,广西南宁530021 [3]广西汇科药物研究所,广西南宁530007

出　　处：《解放军药学学报》2011年第5期444-445,共2页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立白饭树中槲皮素的含量测定方法。方法采用VP-ODS C18(4.6 mm×150 mm,5μm)色谱柱;以甲醇-0.2%磷酸溶液(55∶45)为流动相;流速:1.0 ml/min;检测波长:372 nm。结果白饭树中的槲皮素在0.1002～0.5010μg范围内进样量与峰面积的线性关系良好(r=0.9999);平均回收率为99.25%,RSD为1.16%。结论所建立的方法准确、灵敏、重复性好,可用于白饭树药材的质量控制。Objective To establish a determination method for quercetin in Flueggea virosa（Roxb.ex Willd.） Baill..Methods VP-ODS C18（4.6 mm×150 mm,5 μm）colmnn was used.The mobile phase consisted of methanol-0.2% phosphoric acid solution（55：45）,with the flow rate at 1.0 ml/min.The detection wavelength was at 372 nm.Results The linear relation of quercetin was good in the range of 0.1002-0.5010 μg.The average recovery was 99.25%,and RSD was 1.16%.Conclusion The method is accurate,sensitive and reproducible.It can be applied to the quality control of Flueggea virosa（Roxb.ex Willd.） Baill.

关 键 词：白饭树 槲皮素 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]