子宫内膜异位症患者端粒酶及端粒酶催化亚单位的表达研究  

A research into the expression of telomerase and telomerase reverse transcriptase in patients with endometriosis

作　　者：张凌汝[1] 熊平安[1] 

机构地区：[1]湖北医药学院附属太和医院妇产科,442000

出　　处：《中国优生与遗传杂志》2011年第11期19-21,F0004,共4页Chinese Journal of Birth Health & Heredity

摘　　要：目的探索研究不同分型的子宫内膜异位症患者体内腹腔液中端粒酶及端粒酶逆转录酶的表达及对子宫内膜异位症发展的影响。方法在腹腔镜下收集64例子宫内膜异位症手术患者体内腹腔液,应用酶联免疫吸附实验(ELISA)及荧光原位杂交实验(FISH)方法分别检验收集的腹腔液中端粒酶的阳性表达及端粒酶逆转录酶(hTERC)的扩增情况,并与对照组(盆腔炎患者)相比较。结果应用ELISA方法检验出子宫内膜异位症组腹腔液中端粒酶阳性率与对照组差异无统计学意义;FISH实验检验出子宫内膜异位症组腹腔液中人类端粒酶逆转录酶扩增阳性率0-Ⅱ期与对照组差异无统计学意义;Ⅲ-Ⅳ期患者于对照组差异有统计学意义。结论子宫内膜异位症患者腹腔液中端粒酶逆转录酶表达均高于实验对照组,其可能与异位子宫内膜的恶变及增值活性较高相关。Objective ： To study the expression of different sub - types of telomerase and telomerase reverse transcriptase in the peritoneal fluid of patients with endometriosis, and their impact on endometriosis development： Methods ： Collect peritoneal fluid from 64 patients of endometriosis who were taking laparoscopic surgerys, test the positive expression of telomerase as well as the amplification of telomerase reverse transcriptase （hTERC） by using enzyme -linked immunosorbent assay （ELISA） and fluorescence in situ hybridization （FISH） separately, then, compare the results with the control group （patients with pelvic inflammatory disease）. Results： ELISA test showed that the positive expression rate of telomerase in peritoneal fluid has no significant difference between the endometri- osis group and the control group; FISH test found that the positive amplification rate of telomerase reverse transcriptase （hTERC） in peritoneal fluid has no significant difference between the endometriosis group of stage 0 - Ⅱ and the control group, while in stage Ⅲ-Ⅳ the difference was statistically significant. Conclusion ： Expression of telomerase reverse transcriptase in peritoneal fluid of endometriosis patients is higher than the control group, maybe it is related to the malignant behavior and the high amplification activity of ectooic endometrium.

关 键 词：子宫内膜异位症(EMs) ELISA FISH 端粒酶催化亚单位(hTERC) 端粒酶 

分 类 号：R711.71[医药卫生—妇产科学]