水稻细菌性条斑病菌拮抗细菌的筛选、评价与应用研究  

Screening,Evaluation and Utilization of Antagonistic Bacteria against Xanthomonas oryzae pv.oryzicola

作　　者：张荣胜[1] 刘永锋[1] 陈志谊[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014

出　　处：《中国生物防治学报》2011年第4期510-514,共5页Chinese Journal of Biological Control

基　　金：农业部公益性(农业)行业科研专项(nyhyzx07-056);国家"863"计划现代农业技术领域重大项目(2006AA10A211);江苏省自然科学基金(BK2008350)

摘　　要：从江苏省南京、泰州、扬州和宿迁等地区水稻田采集水稻病叶、健叶、根围土等样品90份,分离纯化得到1173个细菌分离物,对其进行水稻细菌性条斑病菌Xanthomonas oryzae pv.oryzicola皿内抑制试验,共获得12株拮抗能力较强的细菌,其中4株拮抗细菌抑菌圈直径大于27mm。盆栽试验结果表明,12株拮抗能力较强的细菌中有4株对水稻细菌性条斑病菌防效大于50%,其中菌株Lx-11盆栽防效达62.5%。大面积示范试验结果表明菌株Lx-11田间防效达60.2%,显著高于化学药剂20%叶枯唑的防效(51.2%和45.8%)。形态特征、理化特性和分子鉴定分析确定菌株Lx-11为解淀粉芽孢杆菌Bacillus amyloliquefaciens。In this paper, 1173 bacterial strains were isolated fromXanthomonas oryzae pv. oryzicola leaves infected, healthy leaves and rhizosphere of rice plants sampled in Nanjing, Taizhou, Yangzhou and Suqian of Jiangsu province. Among the isolated strains, 12 strains demonstrated high inhibition against X. oryzae pv. oryzicola. Diameter of inhibition zones of 4 strains were more than 27 mm. Control efficiencies of strain Lx-11 were 62.5% and 60.2% in pot and field trials, respectively, significantly higher than those of 20% bismerthlazol treatment （51.2% and 45.8%）. Based on morphological, physiological and biochemical characteristics and 16S rDNA sequence analysis, the strain Lx-11 was identified to be Bacillus amyloliquefaciens.

关 键 词：拮抗细菌 水稻细菌性条斑病菌 防效 解淀粉芽孢杆菌 

分 类 号：S435.111.49[农业科学—农业昆虫与害虫防治] S476.8[农业科学—植物保护]