人皮肤瘢痕成纤维细胞的原代培养及鉴定  

Study on Primary Cultivation and Identification of Human Skin Scar Fibroblasts

作　　者：陈洪[1] 农晓琳[2] 李佳荃[3] CHEN Hong, NONG Xiao-lin, LI Jia-quan （1.Department of Stomatology, Beihai People＇s Hospital, Beihal 536000,China; 2. Department of Oral and Maxillofaeial Surgery, College of Stomatology, Guangxi Medical University, Nanning 530021; 3. Medical Science Research Center, Guangxi Medical University, Nanning 530021,China）

机构地区：[1]北海市人民医院口腔科,广西北海536000 [2]广西医科大学附属口腔医院口腔颌面外科,广西南宁530021 [3]广西医科大学实验中心,广西南宁530021

出　　处：《医学信息》2011年第21期155-156,共2页Journal of Medical Information

摘　　要：目的探讨人皮肤瘢痕成纤维细胞体外培养的方法以及鉴定的方法。方法采用组织块贴壁法进行人皮肤瘢痕成纤维细胞的原代培养．采用免疫细胞化学sP法进行成纤维细胞的鉴定。结果7-12d左右可见成纤维细胞从组织块游出，生长快速，20d左右可连成片，3-5d可传代1次，细胞形态为长梭形。免疫组化染色鉴定抗波形蛋白抗体染色阳性，符合成纤维细胞特性。结论组织块贴壁法可成功培养出的人皮肤瘢痕成纤维细胞。Objective To discuss ~he method of culturing the human skin scar fibroblasts in vitro and the method of identifying the human skin scar fibroblasts. Methods We culture the human skin sear fibroblasts by tissue explant technique. The human skin scar fibroblasts were identified by immunohistoehemical S-P method.Results The fibroblasts cultured by tissue explant technique were observed in 7-12 days and passage of fibroblasts in primary culture and subculture was in 20 days and 3-5 days respectively. The fibroblasts were fusiform shape. Anti-Vimentin ant!body was positive by immunohistochemistry data. The fibroblasts have the characteristics. Conclusion By using the tissue explant technique,we can obtain the human skin scar fibroblasts successfully.

关 键 词：皮肤瘢痕 成纤维细胞 原代培养 鉴定 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学;医药卫生—基础医学]