滨蒿内酯治疗变应性鼻炎的实验研究  

An Experimental Study on Scoparone in Treating Allergic Rhinitis

作　　者：熊瑛 章诗富[2] 成丽兰[2] 姜翠菊[2] 

机构地区：[1]铜川市人民医院耳鼻喉科,陕西铜川727000 [2]广东医学院附属医院耳鼻咽喉头颈外科,广东湛江524023

出　　处：《贵阳医学院学报》2011年第5期452-454,459,共4页Journal of Guiyang Medical College

基　　金：广东省自然基金资助课题(031973)

摘　　要：目的:探讨不同剂量滨蒿内酯(Scoparone,Sco)对变应性鼻炎(AR)大鼠模型的治疗效果。方法:40只雄性SD大鼠,随机分为正常对照组(NC组)、变应性鼻炎组(AR组)、滨蒿内酯10 ml/kg组(Sco A组)、滨蒿内酯20 ml/kg组(Sco B组)和地塞米松组(Dex组),以卵清蛋白致敏和激发方法建立大鼠变应性鼻炎动物模型并给予相应治疗,观察变应性鼻炎大鼠行为学改变,组织病理学方法观察鼻黏膜改变,采用ELISA法检测血清IgE水平。结果:ScoA、ScoB组行为学得分明显低于AR组(6.69±0.88),差异有显著性(P<0.01);Dex组和NC组比较差异无显著性(P>0.05),ScoA、ScoB组鼻黏膜炎性反应明显轻于AR组;ScoA、ScoB组血清IgE中明显低于AR组,差异有显著性(P<0.01);ScoB组血清IgE水平与Dex组和NC组比较差异无显著性(P>0.05),Sco A组血清IgE水平与Sco B、Dex组比较有显著性差异(P<0.05)。结论:滨蒿内酯可抑制大鼠AR的炎症产生,且高浓度(20 ml/kg)效果更好,提示滨蒿内酯对AR有治疗作用。Objective： To observe the therapeutic efficacy of scoparone （Sco） of two different doses on rats with allergic rhinitis （AR）. Methods：Forty male SD rats were randomly divided into 5 groups： normal control group （ group NC ） , AR group （ group AR） , 10 ml/kg Sco treated group （ group Sco A） ,20 ml/kg Sco treated group （group Sco B） ,dexameth treated group （group Dex）. AR model was established by sensitizing SD rats with ovalbumin, and then rats accepted corresponding therapy. Changes of AR rat behaviors were observed. The changes of nasal mucosa were observed with histopathology methods. The levels of serum IgE were determined with enzyme linked immunosorbent assay （ELISA）. Results： The behavior scores of rats in group Sco A and group Sco B were significantly lower than those in group AR（P 〈0.01 ）, and there was no significant difference between groups NC and Dex （ P 〉 0.05 ）. Inflammatory reaction in nasal mucosa of rats in groups Sco A and Sco B was lighter than that in group AR. Serum IgE levels of rats in groups ScoA and Sco B were significantly lower than those in group AR（ P 〈 0.01 ） and there was no significant difference among groups Sco B, Dex and NC （ P 〉 0.05 ）. However, the serum IgE levels of rats in group Sco A were significantly higher than those in groups Sco B and Dex（ P 〈 0.05 ）. Conclusions： Scoparone is effective in suppressing inflammatory reaction of rats with AR, and high concertration of it has better effects suggesting that scoparone should have therapeutic effect in treating AR.

关 键 词：鼻炎 变应性 滨蒿内酯 大鼠 SPRAGUE-DAWLEY 免疫球蛋白E 

分 类 号：R765.213[医药卫生—耳鼻咽喉科]