机械创伤使大鼠心肌对缺血／再灌注损伤的敏感性增加  

Mechanical trauma induces increase of sensitivity of myocardium to ischemia/reperfusion injury in rats

作　　者：温永金[1] 燕子[2] 田珏[3] 王洁[2] 曹成章[1] 梁峰[1] 

机构地区：[1]山西医科大学附属太钢总医院胸心外科,太原030003 [2]山西医科大学基础医学院生理教研室 [3]宁夏医科大学基础医学院病理生理学系

出　　处：《中华创伤杂志》2011年第11期1033-1037,共5页Chinese Journal of Trauma

基　　金：国家自然科学基金资助项目（30872680）

摘　　要：目的观察机械创伤大鼠对心肌缺血／再灌注损伤的敏感性。方法用Noble—Collip创伤仪制备机械创伤模型，采用完全随机分组方法将Wistar大鼠分为假创伤组、创伤组、假创伤＋假手术组、假创伤＋缺血／再灌组和创伤＋缺血／再灌组，于创伤后1周行缺血／再灌注。采用BL-410生物信号记录分析系统记录大鼠左心室收缩压（left ventricular systolic pressure，LVSP）、左心室压力上升和下降最大速率（±dp／dtmax）等心功能数据；采用双抗体夹心ABC—ELISA法检测大鼠血清肌酸激酶同工酶MB（CK—MB）、心肌肌钙蛋白（cTnI）水平；缺血／再灌注后心脏通过伊文思蓝染色、2，3，5-氯化三苯基四氮唑（TTC）复染后，用Image—Pro Plus6．0图像分析软件进行心肌梗死面积测定。结果创伤＋缺血／再灌注组在体心功能明显低于假创伤＋缺血／再灌注组（P〈0．01）；创伤＋缺血再灌注组血清CK—MB和cTnI水平显著高于假创伤＋缺血／再灌注组[CK—MB：（4960±588）ng／nil：（2925±426）ng／ml，P〈0．01；cTnI：（18．10±3．06）ng／ml：（6．67±1．57）ng／ml，P〈0．01]；创伤＋缺血／再灌注组心肌梗死面积明显大于假创伤＋缺血／再灌注组[（36．70±7．42）％：（22．27±4．54）％，P〈0．01]。结论机械创伤使大鼠心肌对缺血／再灌沣棉作的钎戚幛增由n，Objective To observe the sensitivity of myocardium to ischemia/reperfusion （I/R） injury in mechanical trauma rats. Methods The mechanical trauma was established by Noble-Collip drum in the rats that were completely randomized into five groups： sham trauma group, trauma group, sham trauma and sham surgery group, sham trauma and I/R group, trauma and I/R group. The rats were subjected to 30 minutes of isehemia and one hour of reperfusion one week after trauma. The left ventricular systolic pressure （LVSP） and the left ventricular maximum rate of pressure rise and fall （ ± dp/dtmax ） were recorded with BL-410 biological signal recording and analysis system. The levels of serum creatine kinase isoenzyme MB （CK-MB） and cardiac troponin I （cTnI） were detected by double antibody sandwich ABC-ELISA technique. At the end of reperfusion, the heart was excised and stained with Evan＇ s blue dye and triphenyltetrazolium chloride （TTC） to measure the infarct region with Image-Pro Plus 6.0 image analysis software. Results Compared with the sham traumatic I/R group, the cardiac function in vivo was significantly decreased in the traumatic I/R group （ P 〈 0.01 ）. While the serum CK-MB [ （4 960 ±588） ng/ml ：（2 925 ±426） ng/ml, P〈0.01], cTnI [ （ 18. 10 ± 3.06） ng/ml： （6.67 ±1.57） ng/ml, P 〈 0.01 ] levels and myocardial infarct size [ （ 36.70 ±7.42 ） % ： （ 22.27 ±4.54 ） %, P 〈0.01 ] were obviously higher in the traumatic I/R group compared with the sham traumatic I/R group. Conclusion Mechanical trauma increases the sensitivity of myocardium to I/R injury in rats.

关 键 词：胸部损伤 心肌缺血 再灌注损伤 敏感性 

分 类 号：R6[医药卫生—外科学]