附子乙酸乙酯提取物对虚寒模型大鼠肝LA、LDH、PA、Gn含量及ATP酶活力的影响  

Effects of Acetoacetate Extract of Radix Aconite on Hepatic Contents of LA,LDH,PA,Gn,and ATPase Activities in Deficient Cold Model Rats

作　　者：刘一洋[1] 王世军[1] 韩冰冰[1] 刘珊[1] 杨富梅[1] 

机构地区：[1]山东中医药大学基础医学院,济南250355

出　　处：《中国中西医结合杂志》2011年第11期1523-1526,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：科技部973重点基础研究计划资助项目(No.2007CB512601)

摘　　要：目的观察中药附子乙酸乙酯提取物与附子水煎液相比对虚寒模型大鼠能量代谢的影响。方法将Wistar大鼠随机分为空白对照组(空白组,10只)及虚寒模型组(30只)。采用生石膏、龙胆草、黄柏和知母水煎液灌胃大鼠15天复制虚寒模型,之后将虚寒模型组大鼠再随机分为附子乙酸乙酯提取物组(治疗组,10只)、附子水煎液组(对照组,10只)及模型组(10只),两个用药组分别用附子乙酸乙酯提取物和附子水煎液分别灌胃治疗5天,模型组继续予模型药灌胃,空白对照组继续予生理盐水灌胃。检测大鼠肝组织乳酸(LA)、乳酸脱氢酶(LDH)、丙酮酸(PA)、糖原(Gn)含量以及Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶的活力。结果与空白组比较,模型组PA含量、Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活力降低;与模型组比较,两个给药组PA含量升高;与对照组比较,治疗组LDH含量、PA含量、Na+-K+-ATP酶活力升高,差异均有统计学意义(P<0.05)。结论附子乙酸乙酯提取物比附子水煎液热性略大,似乎能产生更多的能量,但是最终结论及具体作用机制有待进一步研究。Objective To observe different effects of acetoacetate extract of Radix Aconite and Radix Aconite Decoction on the energy metabolism in deficient cold model rats. Methods Wistar rats were randomly divided into the blank control group（n=10） and the deficient cold model group（n=30） . The deficient cold rat model was established using decoction consisting of gypsum,Radix Gentianae,Cortex Phellodendri,and Rhizoma Anemarrhenae. The decoction was given to rats of the deficient cold model group by gastrogavage for 5 days. Then these rats were randomly divided into the acetoacetate extract of Radix Aconite group（n=10） ,the Radix Aconite Decoction group（n=10） ,and the model group（n=10） . Rats in the model model group were administered with the decoction by gastrogavage. Rats in the other two groups were administered with the acetoacetate extract of Radix Aconite or Radix Aconite Decoction by gastrogavage for 5 days. The contents of lactic acid（LA） ,lactate dehydrogenase（LDH） ,pyruvate（PA） ,glycogen（Gn） and activities of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase in the hepatic tissue were detected. Results Compared with the blank control group,the PA content,activities of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase decreased in the model group. Compared with the model group,the PA content increased in the other two groups. Compared with the control group,the contents of LDH and PA,and activities of Na＋-K＋-ATPase increased in the the acetoacetate extract of Radix Aconite group with statistical difference（P0.05） . Conclusions The febricity of acetoacetate extract of Radix Aconite was slightly higher than that of Radix Aconite Decoction,seemingly generating more energy. But the final conclusions and concrete mechanisms of action need further studies.

关 键 词：能量代谢 附子提取物 虚寒模型 

分 类 号：R285.5[医药卫生—中药学]