姜黄素对大鼠硒性白内障的影响  

The effects of curcumin on selenic cataract in rats

作　　者：冯万国[1] 付波[2] 杜玲玲[1] 高维奇[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院眼科,150001 [2]大连大学附属新华医院眼科,116021

出　　处：《中华实验眼科杂志》2011年第11期983-986,共4页Chinese Journal Of Experimental Ophthalmology

基　　金：黑龙江省科技攻关计划资助项目（GC06C32803）、哈尔滨市留学归国基金（2006RFLXS025）

摘　　要：背景姜黄素能清除机体中产生的自由基和超氧负离子，从而抑制脂质过氧化反应。研究证实姜黄素具有抗晶状体氧化损伤的作用，但其对年龄相关性白内障的作用尚不清楚。目的观察姜黄素对实验性大鼠硒性白内障的影响。方法选用12日龄健康SD大鼠30只，按随机数字表法将其随机分为空白对照组、模型对照组、姜黄素组，每组10只。除空白对照组外，模型对照组和姜黄素组大鼠均采用皮下注射亚硒酸钠的方法建立硒性白内障模型，并于造模的同时，给予姜黄素组大鼠质量分数0．005％姜黄素灌胃，每131次，共2周。于实验开始后第4、7、10、14天在裂隙灯显微镜下观察各组大鼠晶状体的混浊程度并进行评分。在实验结束后摘出大鼠晶状体，用生化测定法测定晶状体中丙二醛（MDA）及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—Px）的活性。结果空白对照组大鼠晶状体在整个实验过程中保持透明。与模型对照组大鼠比较，姜黄素组大鼠Ⅲ、Ⅳ、V级晶状体混浊的时间明显延迟，差异均有统计学意义（P〈0．05）。实验结束时，3个组MDA含量及SOD、GSH—Px活性的总体比较差异均有统计学意义（MDA：F=215．42，P〈0．01；SOD：F=46．83，P〈0．01；GSH—Px：F=44．29，P〈0．01）。模型对照组、姜黄素组晶状体中SOD的活性明显低于空白对照组，差异均有统计学意义（P〈0．05），但姜黄素组SOD活性明显高于模型对照组（P〈0．05）；模型对照组和姜黄素组晶状体中MDA含量均高于空白对照组（P〈0．01），姜黄素组中MDA含量较模型对照组低（P〈0．01）；模型对照组GSH—Px活性较空白对照组显著下降（P〈0．01），姜黄素组GSH—Px活性较模型对照组高（P〈0．01）。结论姜黄素能显著延缓大鼠硒性白内障的形成过程，但不能抑制硒性白内障的发生发展。姜黄�Background Curcumin can eliminate free radical arising in the system and superoxide anion and further inhibit lipid peroxidation. Researches proved that curcumin has resisting effect on oxidative damage of lens, but its effect on the formation of age-related cataract is under clear. Objective This study was to investigate the effect of curcumin on the cataract induced by sodium selenite. Methods Thirty healthy clean SD rats of 12-day-old were selected. All the rats were randomly divided into the normal control group, model group and curcumin group and ten rats for every group. The selenite cataract models were established in the rats of model group and curcumin group by the injection of sodium selenite subcutaneously. Meanwhile,0. 005% curcumin lavaged the rats in the curcumin group daily for two weeks. Lens of the rats were examined under the slit lamp at 4,7,10,14 days after injection in all groups,and the opacification of lens was graded based on the He＇ criteria. The lenses of rats were obtained at the end of experiment and then the level of superoxide dismutase （SOD） , the activities of malondialdehyde （ MDA ） and glutathione peroxidase（GSH-Px） in rat lens were detected using biochemical assay method. The use of experimental animals followed the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission. Results The lenses were transparent throughout the experimental duration in the blank control group. Compared with model group, the development of the m, rv, v grade of cataract was slow down in the curcumin group with the significant difference（ P〈0.05 ）. The content of MDA,SOD and GSH-Px activity in lens appeared considerable differences among 3 groups （ MDA ： F = 215.42, P〈0.01 ; SOD ： F = 46.83, P〈0.01 ; GSH-Px ： F=44.29,P〈0.01）. The level of SOD was significantly reduced in the model group and the curcumin group compered with blank control group （P〈 0.05 ） and that in the curcumin group was higher than

关 键 词：姜黄素 亚硒酸钠/硒性白内障 超氧化物歧化酶 丙二醛 谷胱甘肽过氧化物酶 

分 类 号：R285.5[医药卫生—中药学]