动态反应器构建组织工程化血管的初步实验研究  

Preliminary experiment of constructing tissue engineering blood vessel by dynamic reactor

作　　者：张燕搏[1] 姜明[2] 王强[1] 吴清玉 胡平[2] 

机构地区：[1]北京协和医学院中国医学科学院阜外心血管病医院小儿心脏中心,北京市100037 [2]清华大学化工系,北京市100086 [3]清华大学第一附属医院心外科,北京市100016

出　　处：《中华实用诊断与治疗杂志》2011年第11期1047-1049,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家自然科学基金(30471721);国家自然科学基金(30670928);国家自然科学基金(81070055)

摘　　要：目的以自行设计的旋转细胞培养器和动态反应器比较静态和动态培养条件下细胞增殖的差异,以期为体外构建组织工程化血管提供依据。方法将兔骨髓间充质干细胞种植在氧等离子体辐照10min聚氨酯膜制成的管状支架上进行细胞培养,比较静态和动态种植、静态和动态培养细胞增殖率的差异。结果自制的旋转培养器、动态反应器工作状态稳定;培养第7,14,28天动态培养组光密度值均高于静态培养组(P<0.01);培养28d甲苯胺蓝染色及扫描电子显微镜观察显示,动态培养组细胞增殖数较静态培养组多、生长更致密,且有顺培养液流动方向排列的趋势。结论动态细胞的种植与培养优于静态。自制的旋转培养器、动态反应器可用于初步的动态培养实验,但仍有待改进。Objective To compare the difference between the static and dynamic culture of the cells by self-designed rotating cell culture and dynamic reactor and to provide the basis for constructing a tissue engineering blood vessel in vitro. Methods Rabbits＇ bone mesenchymal stem cells were implanted in the scaffold of polyurethane treated by 02 plasma for 10 minutes. The proliferation rate of the cells was compared between the static and dynamic groups. Results Self-made rotation reactor and dynamic reactor were suitable for preliminary experiment of dynamic cultivation of the cells. After cultivated for 7, 14 and 28 days, optical density values were higher in dynamic growing group than those in static group, which showed significant differences（P〈0.01）. After 28 days, compared the static and dynamic culture cells by toluidine blue staining and electron microscopy, cells in the static group grew more compact, arranged regularly and aligned in a liquid flow trend. Conclusion Dynamic implantation and cultivation are superior to the static. The self-made rotation reactor and dynamic reactor can be used for preliminary dynamic culture and are to be further improved.

关 键 词：组织工程化血管 旋转培养器 动态反应器 骨髓间充质干细胞 

分 类 号：R318[医药卫生—生物医学工程]