先天性肾积水患儿肾脏水通道蛋白表达与肾实质厚度和肾小球滤过率的相关性  

Correlation of renal AQP1-4 protein expression with renal parenchyma thickness and glomerular filtration rate in children with congenital hydronephrosis

作　　者：李真珍[1] 王志敏[1,2] 邢璐[1,3] 张红[1,4] 文建国[1,3] 

机构地区：[1]郑州大学第一附属医院河南省高等学校临床医学重点学科开放实验室,河南郑州450052 [2]郑州大学第一附属医院内分泌科,河南郑州450052 [3]郑州大学第一附属医院泌尿外科和尿动力学中心,河南郑州450052 [4]郑州大学第一附属医院病理科,河南郑州450052

出　　处：《中国当代儿科杂志》2011年第11期878-882,共5页Chinese Journal of Contemporary Pediatrics

基　　金：国家自然科学基金(批准号:30571931);河南省医学科技攻关计划资助项目(200801002);郑州市技术研究与开发经费支持项目(074SGHH32262)

摘　　要：目的探讨先天性肾积水患儿肾脏水通道蛋白AQP1-4的表达与肾实质厚度和肾小球滤过率(GFR)变化之间的关系。方法利用Western blot检测AQP1-4蛋白在10例先天性肾积水患儿(年龄62.3±18.3个月)10个肾组织和6例来自肾母细胞瘤手术切除患儿的正常肾脏组织(年龄62.7±17.1个月)中的相对表达量。同时对患侧肾脏肾实质厚度和GFR进行评估。积水肾脏AQP1-4表达与GFR以及肾实质厚度之间进行Pearson相关分析检验。结果肾积水组AQP1-4蛋白相对表达均明显低于正常组(P<0.05)。B超测量术前积水侧肾脏肾实质厚度平均为4.59±2.25 mm。99mTc-DTPA测定积水侧肾脏GFR较对侧肾脏明显下降(40±12 mL/min vs 105±20 mL/min,P<0.05)。积水组肾脏中AQP1-4蛋白相对表达量与肾实质厚度之间呈正相关,与患侧肾脏GFR之间亦呈正相关。积水侧肾脏肾实质厚度与GFR之间呈正相关。结论先天性积水患儿肾脏AQP1-4蛋白表达下降,其表达量与肾实质厚度和肾脏GFR的变化呈正相关。[中国当代儿科杂志,2011,13(11):878-882]Objective To study the relationship of renal aquaporin-1,-2,-3,and-4（AQP1-4） expression with renal parenchymal thickness and glomerular filtration rate（GFR） in children with congenital hydronephrotis.Methods Renal tissue samples were obtained from 10 kidneys of 10 children（age： 62.3±18.3 months） with hydronephrosis and who underwent Anderson-Hynes pyeloplasty.Renal control samples were obtained from 6 children（age： 62.7±17.1 months） undergoing nephrectomy for nephroblastoma and were confirmed histologically as normal renal tissues.Renal parenchymal thickness of the hydronephrotic kidneys was measured by ultrasound preoperatively and was verified at operation.Renal GFR was assessed using 99mTc-DTPA scintigraphy preoperatively.Western blot was used to examine the expression of AQP1-4 in the renal tissues.The correlations of renal AQP1-4 expression with the renal parenchymal thickness and GFR were assessed by Pearson correlation analysis.Results The expression of AQP1-4 in the hydronephrotis group was markedly reduced compared to that in the control group（P0.05）.The mean renal parenchymal thickness of the hydronephrotic kidney was 4.59±2.25 mm measured by ultrasound preoperatively.The mean GFR of the obstructed kidney was significantly lower than that of the contralateral kidney in the hydronephrosis group（40±12 mL/min vs 105±20 mL/min;P0.05）.The expression of AQP1,2,3 and 4 was positively correlated with preoperative renal GFR and renal parenchymal thickness in the hydronephrosis group（P0.05）.Renal parenchymal thickness was positively correlated with renal GFR（P0.05）.Conclusions The expression of renal AQP1-4 is reduced in children with congenital hydronephrosis.The expression levels of AQP1-4 are positively correlated with renal parenchymal thickness and GFR.

关 键 词：水通道蛋白 先天性肾积水 肾实质厚度 肾小球滤过率 儿童 

分 类 号：R726.9[医药卫生—儿科]