补肾活血饮对帕金森病大鼠脑内多巴胺D2受体含量的影响  

Effect of Bushen Huoxue Decoction on dopamine D2 receptor in the brain of rats with Parkinson's disease

作　　者：王海明[1] 杨明会[1] 窦永起[1] 刘毅[1] 李绍旦[1] 李敏[1] 

机构地区：[1]解放军总医院中医研究所,北京100853

出　　处：《南方医科大学学报》2011年第11期1879-1881,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(30672762)~~

摘　　要：目的探讨补肾活血饮治疗帕金森病(PD)的作用机制。方法用直接向脑组织内注入6-羟基多巴损毁脑黑质致密部的方法建立PD大鼠模型。将120只SD大鼠随机分为正常对照组、生理盐水模型组和补肾活血饮治疗组,观察各组PD大鼠异常不自主运动变化;用放射免疫法测定治疗后大鼠脑组织多巴胺D2受体(DRD2)平衡解离常数(KD)和最大结合量(Bmax)的变化;免疫组化法观察脑组织DRD2受体阳性细胞数。结果补肾活血饮组大鼠的异常不自主运动较模型组减少。补肾活血饮治疗组大鼠损毁侧脑组织DRD2 Bmax较模型组显著增高(P<0.01),KD值较模型组下降(P<0.01);DRD2受体阳性细胞数(80.9±13.59)较模型组(11.15±6.78)明显升高(P<0.01),但与对照组无显著差异(P>0.05)。结论补肾活血饮能改善PD大鼠不自主运动,促进PD模型大鼠脑组织DRD2表达,提高DRD2亲和力。Objective To explore the mechanism underlying the therapeutic effect of Bushen Huoxue Decoction（BHD）,a traditional Chinese medicinal preparation,on dopamine D2 receptor（DRD2） in the brain of rat models of Parkinson＇s disease（PD）.Methods A total of 120 SD rats were randomized into normal control group,saline model group and BHD-treated group.In the latter two groups,PD rat models were established by direct injection of 6-OHDA to destruct the substantia nigra compact part（SNC） with corresponding treatments.The behavioral changes of the rats were observed.Radioimmunoassay was employed to determine the changes in the equilibrium dissociation constant（Kd） and maximal binding capacity（Bmax） of DRD2,and immunohistochemistry was used to observe the number of the DRD2-positive cells in the brain of the rats.Results BHD can markedly improve the behavioral abnormalities of PD model rats.Compared with those in the saline model group,the Bmax of DRD2 in the damaged hemisphere increased while the Kd of BHD decreased significantly after BHD treatment（P0.01）.The number of DRD2-positive cells in BHD-treated group was significantly higher than that in the model group（80.9±13.59 vs 11.15±6.78,P0.01）,but showed no significant difference from that in the normal control group（P0.05）.Conclusion BHD can improve the behavioral abnormalities and increase the cerebral expression and affinity of DRD2 in PD rat models.

关 键 词：帕金森病 补肾活血饮 多巴胺D2受体 

分 类 号：R285[医药卫生—中药学]