短期苯染毒对大鼠中枢神经系统及血液指标的影响  

Short-term central nervous system symptoms and changes in blood indicators after benzene poisoning in rats

作　　者：刘秋英[1] 梁晓阳[1] 王秀芹[1] 陈念光[1] 孙杰[1] 赖关朝[1] 

机构地区：[1]广东省职业病防治院,广东广州510300

出　　处：《南方医科大学学报》2011年第11期1935-1937,1948,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金(A2007055);广东省职业病防治院专题研究(B200509)

摘　　要：目的观察大鼠在短期苯染毒后的中枢神经系统症状,了解短期苯染毒大鼠血液指标的异常,并探讨这些指标的变化意义。方法取24只成年雌性SD大鼠,随机分成4组(3个实验组,1个对照组),每组各6只,低、中、高剂量组腹腔注射苯,浓度分别39.05、78.11、234.33 mg/kg,对照组为腹腔注射等剂量花生油。观察苯染毒后的中枢神经系统症状,并于染毒24 h后经大鼠股动脉取血,测定各组WBC、RBC、Hb、PLT等血常规指标及ALT、AST、LDH、ALP、BUN、UA等肝肾功能相关指标。结果低、中剂量组大鼠未见明显中枢神经系统异常,高剂量组大鼠出现较明显的中枢神经系统症状。与对照组相比,低、中剂量组血常规指标及肝肾功能相关指标无显著性差别,但高剂量组中WBC、PLT血常规指标及ALT、AST肝功能指标,有显著性差别(P<0.05);各剂量组间、各剂量组与对照组之间的肾功能指标无差异(P>0.05)。结论大鼠在短时间内接触高剂量的苯以后,会出现一定程度的中枢神经系统症状,血常规及肝功能等指标也有一定程度的异常变化,肾功能暂无明显改变。Objective To observe the central nervous system symptoms and alterations in the blood indicators in rats within a short term after benzene poisoning.Method Twenty-four female SD rats were randomized into 4 equal groups to receive intraperitoneal injection of low-,medium-or high-dose benzene（39.05,78.11,and 234.33 mg/kg, respectively） or peanut oil.Blood samples were taken from the rats via the femoral artery 24 h after the injections for routine blood test and liver and kidney function test.Results Intraperitoneal injection of benzene at a high dose,but not at a low or medium dose,caused obvious symptoms in the central nervous system.Benzene either at a low or medium dose did not produce obvious changes in routine blood test or liver and kidney function test as compared with the control group,but a high dose resulted in significant changes in WBC,PLT,ALT and AST（P0.05）. Abnormalities in the renal function were found in none of the groups（P0.05）.Conclusion Exposure to high-dose benzene can result in abnormalities in the central nervous system,routine blood indicators and liver function,but does not obviously affect the kidney function in rats.

关 键 词：苯染毒 SD大鼠 血液指标 肝肾功能指标 中枢神经系统 

分 类 号：R135.12[医药卫生—劳动卫生]