丙烯腈对小鼠肝组织脂质过氧化反应的影响  

Effects of acrylonitrile on lipid peroxidation in the liver tissues of mice

作　　者：马国燕[1] 金娜[1] 李福轮[1] 陈亚[1] 薛红丽[1] 李芝兰[1] 

机构地区：[1]兰州大学公共卫生学院,甘肃兰州730000

出　　处：《毒理学杂志》2011年第5期357-359,360,共4页Journal of Toxicology

基　　金：中央高校基本科研业务费专项资金(lzujbky-2010-140)

摘　　要：目的通过观察丙烯腈(acrylonitrile,AN)对小鼠肝组织脂质过氧化的影响,探讨AN的毒作用机制。方法将200只成年健康SPF级昆明种雄性小鼠按体重随机分为4组,分别为阴性对照(生理盐水)组和低(1.25 mg/kg)、中(2.50 mg/kg)、高(5.00 mg/kg)剂量AN染毒组,每组50只。采用腹腔注射方式进行染毒,染毒容量为10 ml/kg,1次/d,连续染毒5d。于首日染毒后第7、14、21、28和35天测定其肝组织中丙二醛(MDA)含量、过氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力。结果 AN首日染毒后第28天中、高剂量组MDA含量与阴性对照组比较明显升高,有统计学意义(P<0.05,P<0.01),第35天高剂量组MDA含量高于阴性对照组,有统计学意义(P<0.01);第28天高剂量组SOD活力高于阴性对照组(P<0.01);第28天组高剂量组GSH-Px活力显著高于阴性对照组和中剂量组(P<0.01,P<0.05)。结论 AN可能通过破坏氧化-抗氧化体系的平衡,诱导小鼠肝组织产生氧化损伤,在首日染毒后第28天时比较明显。Objective To explore the toxicity mechanism of acrylonitrile（AN） by observing the lipid peroxidation of the liver tissues in mice.Methods Two hundred adult male mice（6～8 weeks old,SPF grade） were randomly divided into 4 groups according to the body weight,the negative control group（normal saline）,the low-dose AN group（1.25 mg/kg）,the medium-dose AN group（2.50 mg/kg）,and the high-dose AN group（5.00 mg/kg）.All mice were treated via daily intraperitoneal injection for 5 successive days.Malonaldehyde（MDA） concentration and the activities of superoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） in the liver tissues were measured on the 7th,14th,21st,28th,and 35th day after the first treatment.Results In comparison with different dose groups within the same period of time showed that the concentrations of MDA increased significantly in medium-and high-dose groups at 28th days（P0.05,P0.01,respectively）,and the concentration of MDA was significantly higher in high-dose group than that in the negative control group at 35th day（P0.01）;Compared to the negative control group,the SOD activity of liver tissues increased significantly in high-dose group（P0.01） and the GSH-Px activity was significantly higher in high-dose group than those in the negative control group and medium-dose group at 28th day（P0.01,P0.05,respectively）.Conclusion It suggested that AN might impair the balance of antioxidant system,induce lipid peroxidation damage to mice liver,and that was significantly at 28th day.

关 键 词：丙烯腈 脂质过氧化 小鼠 

分 类 号：R114[医药卫生—卫生毒理学]