黄芪皂苷提取纯化工艺研究  

Research on Technology of Extraction and Purification of Astragaloside

作　　者：杨明[1,2] 赵强强[1] 熊永爱[1] 王淼[1] 韩丽[1] 谢兴亮[3] 

机构地区：[1]成都中医药大学中药材标准化教育部重点实验室,四川成都611137 [2]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004 [3]成都医学院,四川成都610083

出　　处：《成都中医药大学学报》2011年第3期44-46,共3页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家科技支撑计划项目(2006BA09B08-14);国家重大新药创制科技专项(2009ZX09103-307)

摘　　要：目的:通过对黄芪皂苷提取纯化工艺的研究,确定了最佳的提取纯化工艺条件。方法:采用正交试验进行了乙醇提取工艺的研究,再采用大孔吸附树脂法进行了纯化工艺研究。结果:以6倍量60%乙醇提取3次,每次1 h条件下黄芪皂苷的提取量较高,提取液上树脂柱后先以水洗除去杂质,再收集全部70%乙醇洗脱液即为黄芪总皂苷的有效部位。结论:采用此工艺可以达到黄芪药材的提取、纯化的目的,为其工业化生产应用奠定了科学的基础。Objective：Through Radix Astragali material＇s extraction and purification of the study to determine the best conditions for the extraction and purification process.Methods：Orthogonal test that the alcohol having been in progress extracts the handicraft research.Adopt macroporous resin method on the purification process again.Results：60% Alcohol extracts a triple,time is one hour,and increase the volume of alcohol（6times）under the conditions.The content of Astragaloside is higher.After the first washing to remove impurities,then collect all the 70%ethanol eluate,which is the effective parts of Astragaloside.Conclusion：This process can be achieved Radix Astragali ＇s extraction and purification of the purpose of their industrial production for the application of the scientific basis.

关 键 词：黄芪 黄芪皂苷 大孔吸附树脂 正交试验 

分 类 号：O6-332[理学—化学]