滨蒿内酯鉴别迭鞘石斛可行性的探讨  

Study on Feasibility of Identification for Dendrobium denneanum Kerr.with Scoparone

作　　者：周颖[1] 夏厚林[1] 彭颖[1] 张廷模[1] 程军[1] 黄立华[1] 

机构地区：[1]成都中医药大学药学院,四川成都611137

出　　处：《成都中医药大学学报》2011年第3期51-52,61,共3页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：四川省科技支撑计划项目(No.2009FZ0101)

摘　　要：目的:探讨迭鞘石斛薄层鉴别方法的可行性。方法:以HPLC法DAD扫描确认迭鞘石斛中是否含有滨蒿内酯。结果:在迭鞘石斛薄层鉴别试验中,供试品色谱在与对照品色谱相应的位置上有斑点,但斑点颜色与滨蒿内酯对照品的颜色有差异;在HPLC鉴别试验中,滨蒿内酯检测限为2.14 ng时,迭鞘石斛供试品中出现与滨蒿内酯相应的色谱峰,但它们色谱峰的光谱图不相同。结论:迭鞘石斛药材标准中以滨蒿内酯为对照品的鉴别方法值得商榷和完善。Objective：Study on the feasibility of TLC identification method for Dendrobium denneanum Kerr.Methods： HPLC-DAD scan was used to examine the possible existence of scoparone in Dendrobium denneanum Kerr.Results：The spot obtained from the Dendrobium denneanum Kerr.corresponded to that obtained from scoparone in position but not in color by TLC.Correspongding chromatograph peak was shown in Dendrobium denneanum Kerr.by HPLC when the detection limit of scoparone was 2.14 ng,but spectrograms of their chromatograph peaks were different.Conclusion：The identification method with scoparone as reference substance should be discussed and improved in the medicinal material standard of Dendrobium denneanum Kerr.

关 键 词：迭鞘石斛 滨蒿内酯 薄层鉴别 HPLC 

分 类 号：R917[医药卫生—药物分析学] O657.7[医药卫生—药学]