外源hTERT基因对人肝细胞增殖及CYP450 19A1表达的影响  

Effect of exogenous human telomerase reverse transcriptase gene on proliferation of human hepatocytes and expression of CYP450 19A1

作　　者：王开利[1] 迟淑萍[2] 孙杰[2] 邢汉前[1] 刘俊威[1] 赵鸿[1] 赵军[1] 

机构地区：[1]解放军第302医院血液净化科,北京100039 [2]解放军第302医院传染病研究所免疫室,北京100039

出　　处：《军医进修学院学报》2011年第11期1137-1138,1163,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金面上项目(30671849)~~

摘　　要：目的探讨外源hTERT基因对体外培养的人肝细胞增殖及细胞色素P450 19A1(CYP450 19A1)表达的影响。方法将携带有hTERT片段的重组逆转录病毒载体pLNCX2-hTERT转染至体外培养的人肝细胞;四甲基偶氮唑蓝比色(MTT)法观察hTERT表达对细胞增殖的影响;通过采用Western blot法检测CYP450 19A1的表达情况。结果成功将带有hTERT逆转录病毒载体pLNCX2-hTERT转染至人肝细胞,转染后的肝细胞组较未转染组增殖明显,转染前后肝细胞在55kU处均可见有CYP450 19A1蛋白表达。结论外源hTERT基因可促进肝细胞增殖;转染组与未转染组肝细胞均可表达CYP450 19A1。Objective To study the effect of human telomerase reverse transcriptase（hTERT） gene on proliferation of in vitro cultured human hepatocytes and expression of cytochrome P450 19A1.Methods Recombinant retrovirus vector carrying pLNCX2-hTERT fragment was transfected into in vitro cultured human hepatocyte.Effect of hTERT gene on proliferation of human hepatocytes and expression of P450 19A1 was detected by methyl thiazol tetrazolium（MTT） and Western blot,respectively.Results The retrovirus vector carrying pLNCX2-hTERT fragment was successfully transfected into human hepatocytes.The proliferation of human hepatocytes was significantly faster after transfection than before transfection.Hepatocytes at 55kU expressed CYP450 19A1 protein before and after transfection.Conclusion Exogenous hTERT gene can promote the proliferation of human hepatocytes,both transfected or untransfected hepatocytes can express CYP450 19A1.

关 键 词：基因 RNA指导的DNA聚合酶 肝细胞 增殖 细胞色素P450酶系19A1 

分 类 号：R34[医药卫生—基础医学]