重组人生长激素对严重烫伤大鼠早期心肌组织MPO活性的影响  

Effect of recombinant human growth hormone on early activities of myocardial myeloperoxidase in rats with scalded injury

作　　者：朱国玲[1] 门秀丽[2] 樊学东[1] 刘朝永[1] 邱方[3] 

机构地区：[1]河北联合大学附属开滦医院,唐山063000 [2]河北联合大学,唐山063000 [3]河北联合大学附属医院,唐山063000

出　　处：《军医进修学院学报》2011年第11期1152-1154,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的观察重组人生长激素(rhGH)对严重烫伤大鼠早期心肌组织髓过氧化物酶(MPO)活性的影响。方法 SPF级SD大鼠60只,雄性,随机分为对照组(B组,n=30),治疗组(R组,n=30)。复制30%TBSAⅢ°烫伤模型,伤后B组按Parkland公式补液,R组在B组补液基础上于伤后6h始予rhGH 3U/(kg.d)皮下注射,两组均于烫伤后各时相点:0、12、24、48、96h检测血清心肌肌钙蛋白I(cTnI)含量和心肌组织MPO活性。结果 B组伤后不同时相点cTnI含量及MPO活性显著升高(P<0.01),伤后12h升高最为显著。心肌MPO活性升高与cTnI水平存在显著正相关(r=0.969,P<0.01)。R组相应时相点cTnI含量及MPO活性与B组趋势一致,但较B组显著升高(P<0.05或0.01);病理切片显示:R组较B组炎细胞浸润、心肌损伤均有所加重。结论严重烫伤后早期大鼠存在局灶性心肌炎,早期应用rhGH加重了烫伤后大鼠心肌损伤。Objective To study the effect of recombinant human growth hormone（rhGH） on early activities of myocardial myeloperoxidase in rats with scald.Methods Sixty male Sprague-Dawley rats were randomly divided into control group and treatment group,30 in each group.A 30% TBSA III scald model was established.Rats in control group received fluid infusion after scald according to the formula of Parkland,and those in treatment group received fluid infusion with rhGH（3U/kg·d） subcutaneously injected 6h after scald.Serum cTnI level and myocardial myeloperoxidase activities in both groups were measured at 0,12,24,48,and 96h after scald.Results The serum cTnI level and myocardial myeloperoxidase activities were significantly higher in control group B at different time points after scald,especially 12h after scald（r=0.969,P〈0.01）.The same trends in serum cTnI level and myocardial myeloperoxidase activites was observed in treatment group.However,they were significantly higher than those in control group（P〈0.05）.Pathological examination showed that inflammatory cell infiltration and myocardial damage were severer in treatment group than in control group.Conclusion Focal myocarditis occurs in rats early after severe scald,and early use of rhGH can aggravate myocardial damage.

关 键 词：烫伤 心肌损伤 生长激素 髓过氧化物酶 

分 类 号：R541.9[医药卫生—心血管疾病] R544[医药卫生—内科学]