排除主效作用下水稻产量性状QTL的检测  

QTL ANALYSIS FOR YIELD TRAITS OF RICE IN EXCLUSION OF MAJOR EFFECT

作　　者：钟娟[1,2] 刘文强[1,3] 吴建利[2] 贺浩华[1] 庄杰云[2] 

机构地区：[1]江西农业大学作物生理生态与遗传育种教育部重点实验室,江西南昌330045 [2]中国水稻研究所水稻生物学国家重点实验室/国家水稻改良中心,浙江杭州310006 [3]湖南省农业科学院水稻研究所,湖南长沙410125

出　　处：《核农学报》2011年第5期857-862,共6页Journal of Nuclear Agricultural Sciences

基　　金：高等学校博士学科点专项科研资金资助(20070410002);江西省重大科技创新项目(20061G0100300);十一五国家粮食丰产科技工程项目江西分析(2006BAD02A04);国家863计划项目(课题编号2009AA101101)

摘　　要：本研究旨在排除主效QTL效应的基础上检测控制水稻产量性状的微效QTL。前期应用中156/谷梅2号重组自交系(RIL)群体在第7染色体RM2-RM214区间上检测到控制抽穗期和产量性状的主效QTL,本研究挑选在此区间呈谷梅2号基因型的两个株系,配组衍生新的RIL群体,检测控制水稻产量性状的QTL。共检测到25个产量性状QTL,其中,8个QTL前期在中156/谷梅2号RIL群体亦呈显著作用,加性效应方向与前期一致;6个QTL位于其中2个共有区间中,但呈显著的性状从原来的单个性状增加到4个性状;11个QTL所处的区间,前期均未检测到产量性状QTL。前期检测到QTL中有2个在本研究中未检测到,其中1个在RM2-RM214区间为谷梅2号背景下不发挥作用,另一个在前期研究中对表型方差的贡献率仅为0.55%。两个群体QTL检测结果的比较结果表明,排除主效QTL可极大地提高微效QTL得到检测的几率。This study was conducted to detect quantitative traits loci（QTL） for yield traits in the exclusion of major QTLs.In a previous mapping of QTLs for heading date and yield traits using Zhong 156/Gumei 2 recombinant inbred line（RIL） population of rice,a major QTL was located in the interval RM2-RM214 on chromosome 7.Two lines showing the Gumei 2 genotype in this interval were selected and crossed to derive a new RIL population.A total of 25 QTLs were detected,of which 8 also had significant effect in the previous study and the direction of the additive effect always remained unchanged,6 more were located in two of the common QTL regions but significant effects were detected for 3 more traits in each region,respectively.The remaining 11 QTLs were located in regions where no QTL for yield traits were detected in the previous study.Two QTLs detected in the previous population was not detected in the present study,of which one had been shown to exhibit no effect in the background of Gumei 2 genotype in the RM2-RM214 interval,and the other had a minor contribution of 0.55% to the phenotypic variance in the previous study.Comparison between the two RIL populations showed that efficiency of the QTL detection could be greatly improved by eliminating the effect of major QTL.

关 键 词：水稻 数量性状座位 重组自交系 产量性状 

分 类 号：S511[农业科学—作物学]