十字花科植物C2H2型锌指蛋白新基因BcMF20同源序列克隆与进化分析  

CLONING AND EVOLUTIONARY ANALYSIS OF HOMOLOGOUS SEQUENCES OF A NOVEL GENE ENCODING C2H2 ZINC FINGER PROTEIN IN CRUCIFERAE

作　　者：韩莹琰[1,2] 张爱红[1] 范双喜[2] 曹家树[1] 

机构地区：[1]浙江大学蔬菜研究所,浙江杭州310029 [2]北京农学院植物科学技术学院,北京102206

出　　处：《核农学报》2011年第5期916-921,共6页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(30871715);浙江省重大科技项目(2010C12004);北京市教委科技项目(KM201110020004);北京市科技新星项目(2010B020)

摘　　要：为获取C2H2型锌指蛋白基因在物种间的分类和进化关系,分析其在基因结构上的保守性,更好地研究该基因在植物花粉发育中的功能,根据白菜花粉发育相关基因BcMF20 DNA序列设计引物,运用PCR技术分别从十字花科芸薹属和萝卜属18种材料中克隆了C2H2型锌指蛋白基因BcMF20的同源序列。经序列比对分析,BcMF20同源基因在DNA序列上的相似性为86.9%～100%,所推导的氨基酸序列相似性为77.6%～100%,在锌指的保守区域,氨基酸序列完全相同。这些结果表明该基因具有较好的保守性,可能在十字花科植物的花粉发育中行使重要的功能。In order to elucidate categorization evolvement of C2H2 zinc finger protein,and clarify mechanism on C2H2 zinc finger protein in pollen development among plants at molecular level,the genes encoding C2H2 zinc finger protein analogues from 18 species of genera Brassicaand Raphanusin Cuciferae were obtained by PCR strategy using specific primers designed from the full length of BcMF20,a putative gene encoding C2H2 zinc finger protein which was related to the male sterility.The phylogenetic relationships of these species belonging to the family Cruciferae were investigated through comparison of the sequences.Homologous sequences of BcMF20comparison indicated that the similarities among the genes at nucleotide and amino acid levels were 86.9% ~ 100% and 77.6%～100%,respectively.In the zinc finger regions of homologous sequences,the amino acid sequences were identical.These results showed that the BcMF20was relative conservation in evolution in Cruciferae,and BcMF20may play an important role in pollen development.

关 键 词：十字花科 C2H2锌指蛋白 BcMF20 克隆 系统发育 

分 类 号：S184[农业科学—农业基础科学]