芥菜Rubisco小亚基的基因克隆及其在芜菁花叶病毒侵染后的表达分析  

CLONING OF RUBISCO SMALL SUBUNIT GENE FROM MUSTARD AND ANALYSIS OF ITS EXPRESSION IN RESPONSE TO TURNIP MOSAIC VIRUS INFECTION

作　　者：朱磊[1] 杨景华[1] 张明方[1] 

机构地区：[1]浙江大学园艺系园艺植物遗传资源与功能改良开放实验室农业部园艺植物生长发育与品质调控重点开放实验室,浙江杭州310029

出　　处：《核农学报》2011年第5期945-950,共6页Journal of Nuclear Agricultural Sciences

基　　金：教育部新世纪优秀人才支撑计划(J20070206)

摘　　要：以耐病性叶用芥菜为材料,克隆和分析了芥菜Rubicso小亚基的基因序列,研究了芜菁花叶病毒(Turnip mosaic virus,TuMV)侵染对Rubicso小亚基基因表达的影响。结果显示:Rubicso小亚基基因全长为842bp,其开放阅读框(ORF)可编码181个氨基酸,其中包括2个α螺旋及4个β折叠二级结构,并含有一些高度保守的氨基酸残基。接种TuMV后,随着上部第3片真叶中外壳蛋白基因的逐渐积累,叶片的正常生长受到抑制,同时Rubicso小亚基的表达呈现先上升后下降的趋势,表明其参与了芥菜对TuMV侵染的响应。We cloned the cDNA encoding the Rubisco small subunit of leafy mustard and studied its expression after Turnip mosaic virus（TuMV） infection.The result showed that the full-length cDNA of Rubisco small subunit gene was 842bp and contained an open reading frame（ORF） encoding 181 amino acids,which had two α-helixes,four β-sheets,and some conserved residues.With the accumulation of CP in upper systemic leaves after TuMV infection,mustard growth was impaired.At the same time,expression of Rubisco small subunit was increased before 6 days of inoculation and then gradually decreased,which suggested that the Rubisco small subunit participated in the response of mustard to TuMV infection.

关 键 词：芥菜 RUBISCO小亚基 抗病性 叶绿素荧光 TUMV 

分 类 号：S436.344[农业科学—农业昆虫与害虫防治]