不同生长素类型及ABA搭配对小麦幼胚再生效果的影响  

EFFECTS OF DIFFERENT AUXINS AND COMBINING APPLICATION WITH ABA ON REGENERATION OF IMMATURE EMBRYOS OF WHEAT

作　　者：别晓敏[1] 杜丽璞[1] 佘茂云[1] 徐惠君[1] 叶兴国[1] 

机构地区：[1]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程/农业部作物遗传育种重点开放实验室,北京100081

出　　处：《核农学报》2011年第5期1023-1028,共6页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(30971776);国家转基因研究专项(2008ZX08010-004)

摘　　要：生长素是小麦幼胚产生胚性愈伤组织的关键成分,培养基中适宜的生长素种类、浓度和组合决定着小麦幼胚再生效果。本研究以小麦基因型CB037幼胚为材料,在分别确定dicamba、2,4,5-T和picloram的适宜用量的基础上,比较了2,4-D、dicamba、2,4,5-T、picloram在适宜浓度下诱导再生的效果,筛选出ABA搭配2,4-D的最佳浓度组合。结果表明,dicamba、2,4,5-T和picloram的适宜用量分别为2.5mg/L、3.0mg/L和3.0mg/L,2,4-D诱导小麦幼胚再生效果最好,其次为dicamba,picloram和2,4,5-T诱导效果较差;在含有2,4-D 2.0mg/L的培养基中加入ABA 0.3mg/L能明显抑制幼胚直接萌发,显著提高胚性愈伤组织诱导率和再生率。It is necessary to improve plant regeneration efficiency for cell and genetic engineering breeding of wheat.To investigate appropriate type and concentration of auxins（dicamba,2,4,5-T,picloram） and combination of 2,4-D and ABA for wheat immature embryo culture, a new wheat line CB037 was applied to investigate the influence of three auxins and combination effect of 2,4-D and ABA.Results showed that the optimal concentrations of dicamba,2,4,5-T,picloram when used alone were 2.5mg/L,3.0mg/L and 3.0mg/L,respectively.Compared to 2,4-D with the highest efficiencies in both of callus differentiation and green shoots induction,dicamba was the most suitable auxin to induce embryogenic callus other than to induce regeneration shoots,and 2,4,5-T and picloram followed the above two auxins either in embryogenic callus induction or green shoots induction.In the presence of 2.0mg/L 2,4-D and 0.3mg/L ABA in the callus induction medium,the best regeneration efficiency from the immature appeared,improving the callus quality and the green seedling numbers dramatically.

关 键 词：小麦 幼胚 生长素 胚性愈伤组织诱导率 植株再生率 

分 类 号：S512.1[农业科学—作物学]