应用微流控电泳联合检测载脂蛋白C3基因型  

Application of Microfluidic Chip Electrophoresis in Combining Detection of Apolipoprotein C3 Promoter Genotype

作　　者：俞娟[1] 卢美红[2] 王惠民[2] 仲人前[3] 

机构地区：[1]南通大学附属医院检验医学中心江苏南通,226001 第二军医大学附属长征医院实验诊断科上海200003 [2]南通大学附属医院检验医学中心,江苏南通226001 [3]第二军医大学附属长征医院实验诊断科,上海200003

出　　处：《现代检验医学杂志》2011年第5期87-90,共4页Journal of Modern Laboratory Medicine

基　　金：南通大学附属医院基金（Tdfy0929）.

摘　　要：目的 建立应用微流控电泳联合检测载脂蛋白C3（APOC3）启动子区2种基因型的方法.方法 采用聚合酶链反应（PCR）技术扩增APOC3基因启动子区包含T-455C和C-482T多态性在内的DNA片段,产物分别用限制性内切酶BseGⅠ和MspⅠ酶切后同时进行微流控电泳,分析APOC3基因启动子区这2位点的基因型.结果 成功联合检测APOC3启动子区C-482T和T-455C多态性基因型.结论 应用微流控电泳联合检测APOC3启动子区2位点基因型方法可行,适合应用于常规实验或科研工作.Objective To establish an assay for detection of 2 polymorphisms of the apolipoprotein C3 （APOC3） gene promoter by application of microfluidic chip etectrophoresis simultaneously. Methods Polymerase chain reactlon（PCR） was performed to amplify APOC3 including T-455C and C-482T polymorphism,PCR production was digested with restriction endonuclease BseG I and Msp I respectively,APOC3 genotype were analyzed after microfluidic chip electrophoresis. Results PCR-RFLP in combination with microfluldic chip electrophoresis was established to analyze the C-482T and T-455C polymorphisms of APOC3 gene promotor at the same time. Conclusions It is feasible to detect 2 polymorphlsms of APOC3 at the same time by applying the microfluidic chip electrophoresis and the method is suitable for the routine or study work in ordinary laboratory.

关 键 词：载脂蛋白C3 基因多态性 基因型 微流控电泳 

分 类 号：Q786[生物学—分子生物学]