用DIGFA,ELISA两种免疫诊断方法检测包虫病的综合评价  

Comprehensive Evaluation of Detection Echinococcosis with DIGFA and ELISA Methods

作　　者：欧仕颖 余长芳 

机构地区：[1]四川省甘孜州人民医院检验科,四川康定626000

出　　处：《现代检验医学杂志》2011年第5期121-123,共3页Journal of Modern Laboratory Medicine

摘　　要：目的 探讨斑点免疫胶体金渗滤法与ELISA法检测包虫抗体的相关性及免疫诊断价值.方法 用四种抗原联合检测的DIGFA法分别检测感染包虫病患者、健康人群、疑为包虫病感染者血清中的特异性抗体,并与常规ELISA法进行平行检测和比较.结果 DIGFA法检测包虫病患者血清中特异性抗体的敏感度为96.1% （74/77）、特异度为100%（20/20）,与ELISA 法检测结果符合率为93.7%;不同虫荷数感染者间抗体水平差异无统计学显著性意义（P〉0.05）.结论 用四联抗原检测感染包囊虫患者血清中特异性抗体具有较好的敏感度和特异度,组间差异无显著性.DIGFA法与ELISA法相比,前者更为简便、快速,无需特殊仪器设备,可单份检测,适合基层寄生虫病的临床检验和流行病学调查.若能进一步纯化检测用抗原,囊型抗原和泡型抗原间的特异性,则更具有非常大的潜在应用价值.Objective To explore jinbiao immune filtration method with rapid detection antibody specificity hydatid with ELISA test hydatid antibody and immune diagnostic value. Methods With four kinds of antigen detection method of DIG- FA respectively,healthy people infected echinococcosis,suspected patients infected blood serum antibodies echinococcosis and specific conventional ELISA for parallel detection and compared. Results DIGFA method to detect echinococcosis pa- tients serum antibodies sensitivity of 96.1% specificity （74/77）,specific 100% （20 /20） testing results as ELISA,Differ- ent insects infected antibody levels between the number of Hollywood had no significant difference （P^0. 05）. Conclusion With four league antigen detection infected patients serum bag cysticercosis specific antibodies had good sensitivity and specificity,there was no significant difference between groups. Compared with ELISA DIGFA method the former is more convenient,quick,without special equipment ,could a single copy detection,suitable for parasites clinical and epidemiological survey. If could further purification with antigen detection,capsule antigens and bubble antigens,between the specificity of more great potential applications value.

关 键 词：包虫病 斑点金免疫渗滤法 酶联免疫吸附试验 综合评价 

分 类 号：R383.33[医药卫生—医学寄生虫学] R446.61[医药卫生—基础医学]